LOCUS       sequence-157959-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tissue-specific multiplex gRNA plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Port F, Bullock SL
  TITLE     Augmenting CRISPR applications in Drosophila with tRNA-flanked 
            sgRNAs.
  JOURNAL   Nat Methods. 2016 Oct;13(10):852-4. doi: 10.1038/nmeth.3972. Epub 
            2016 Sep 5.
  PUBMED    27595403
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3972"; journalName: "Nat Methods"; date: "2016-10"; 
            volume: "13"; issue: "10"; pages: "852-4"
COMMENT     Sequence Label: pCFD6
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        403..478
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ggcgcggggt ggctgagagc atcagttgtg aatgaatgtt cgagccgagc agacgtgccg
       61 ctgccttcgt taatatcctt tgaataagcc aactttgaat cacaagacgc ataccaaacg
      121 aattcgggct ttgagtgtgt gtagacatca agcatcggtg gttcagtggt agaatgctcg
      181 cctgccacgc gggcggcccg ggttcgattc ccggccgatg cagggtcttc gtttcagagc
      241 tatgctggaa acagcatagc aagttgaaat aaggctagtc cgttatcaac ttgaaaaagt
      301 ggcaccgagt cggtgctaac aaagcaccag tggtctagtg gtagaatagt accctgccac
      361 ggtacagacc cgggttcgat tcccggctgg tgcagaagac ctgttttaga gctagaaata
      421 gcaagttaaa ataaggctag tccgttatca acttgaaaaa gtggcaccga gtcggtgcta
      481 acaaagcacc agtggtctag tggtagaata gtaccctgcc acggtacaga cccgggttcg
      541 attcccggct ggtgcatcta gaggatcttt gtgaaggaac cttacttctg tggtgtgaca
      601 taattggaca aactacctac agagatttaa agctctaatt aaaataacaa atcaattgtt
      661 ttataatatt cgtacgattc tttgattatg taataaaatg tgatcattag gaagattacg
      721 aaaaatataa aaaatatgag ttctgtgtgt ataacaaatg ctgtaaacgc cacaattgtg
      781 tttgttgcaa ataaacccat gattatttga ttaaaattgt tgttttcttt gttcatagac
      841 aatagtgtgt tttgcctaaa cgtgtactgc ataaactcca tgcgagtgta tagcgagcta
      901 gtggctaacg cttgccccac caaagtagat tcgtcaaaat cctcaatttc atcaccctcc
      961 tccaagttta acatttggcc gtcggaatta acttctaaag atgccacata atctaataaa
     1021 tgaaatagag attcaaacgt ggcgtcatcg t
//
