LOCUS       sequence-157975-        1020 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses PCYT2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1020)
  AUTHORS   Chidley C, Trauger SA, Birsoy K, O'Shea EK
  TITLE     The anticancer natural product ophiobolin A induces cytotoxicity by 
            covalent modification of phosphatidylethanolamine.
  JOURNAL   Elife. 2016 Jul 12;5. pii: e14601. doi: 10.7554/eLife.14601.
  PUBMED    27403889
REFERENCE   2  (bases 1 to 1020)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2016-07-12"; volume: "5. pii: e14601. doi"; pages: " 
            10.7554/eLife.14601"
COMMENT     Sequence Label: pLJM1-PCYT2
FEATURES             Location/Qualifiers
     source          1..1020
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             57..80
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctatataagc agagctggtt tagtgaaccg tcagatccgc tagcgctacc ggtatggact
       61 acaaggatga cgatgacaag ggtggtggtg gtggtgcgat gatccggaac gggcgcgggg
      121 ctgcaggcgg cgcagagcag ccgggcccgg ggggcaggcg cgccgtgagg gtgtggtgcg
      181 atggctgcta tgacatggtg cattacggcc actccaacca gctgcgccag gcacgggcca
      241 tgggtgacta cctcatcgta ggcgtgcaca ccgatgagga gatcgccaag cacaaggggc
      301 ccccggtgtt cactcaggag gagagataca agatggtgca ggccatcaaa tgggtggacg
      361 aggtggtgcc agcggctccc tacgtcacta cactagagac cctggacaaa tacaactgtg
      421 acttctgtgt tcacggcaat gacatcaccc tgactgtaga tggccgggac acctatgagg
      481 aagtaaagca ggctgggagg tacagagaat gcaagcgcac gcaaggggtg tccaccacag
      541 acctcgtggg ccgcatgctg ctggtaacca aagcccatca cagcagccag gagatgtcct
      601 ctgagtaccg ggagtatgca gacagttttg gcaagtgccc tggtgggcgg aacccctgga
      661 ccggggtatc ccagttcctg cagacatctc agaagatcat ccagtttgct tctgggaagg
      721 agccccagcc aggggagaca gtcatctatg tggctggtgc cttcgacctg ttccacatcg
      781 ggcatgtgga cttcctggag aaggtgcaca ggctggcaga gaggccctac atcatcgcgg
      841 gcttacactt tgaccaggag gtcaatcact acaaggggaa gaactacccc atcatgaatc
      901 tgcatgaacg gactctgagc gtgctggcct gccggtacgt gtcagaagtg gtgattggag
      961 ccccgtacgc ggtcacagca gagctcctaa gtcacttcaa ggtggacctg gtgtgtcacg
//
