LOCUS       sequence-158066-         796 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid for constitutive or doxycycline-inducible expression of 
            H129N nuclease-dead mutant of human MRE11. Confers resistance to 
            puromycin. Use T-REx cells for doxycycline-inducible expression..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 796)
  AUTHORS   Chanut P, Britton S, Coates J, Jackson SP, Calsou P
  TITLE     Coordinated nuclease activities counteract Ku at single-ended DNA 
            double-strand breaks.
  JOURNAL   Nat Commun. 2016 Sep 19;7:12889. doi: 10.1038/ncomms12889.
  PUBMED    27641979
REFERENCE   2  (bases 1 to 796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-09-19"; volume: "7:12889. doi"; pages: " 
            10.1038/ncomms12889"
COMMENT     Sequence Label: pICE-HA-MRE11-H129N
FEATURES             Location/Qualifiers
     source          1..796
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             176..202
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg caagcttgcg gccgccgcca ccatgtaccc
      181 ctacgatgtg cctgactacg ccaccggtat gagtactgca gatgcacttg atgatgaaaa
      241 cacatttaaa atattagttg caacagatat tcatcttgga tttatggaga aagatgcagt
      301 cagaggaaat gatacgtttg taacactcga tgaaatttta agacttgccc aggaaaatga
      361 agtggatttt attttgttag gtggtgatct ttttcatgaa aataagccct caaggaaaac
      421 attacatacc tgcctcgagt tattaagaaa atattgtatg ggtgatcggc ctgtccagtt
      481 tgaaattctc agtgatcagt cagtcaactt tggttttagt aagtttccat gggtgaacta
      541 tcaagatggc aacctcaaca tttcaattcc agtgtttagt attcatggca ataatgacga
      601 tcccacaggg gcagatgcac tttgtgcctt ggacatttta agttgtgctg gatttgtaaa
      661 tcactttgga cgttcaatgt ctgtggagaa gatagacatt agtccggttt tgcttcaaaa
      721 aggaagcaca aagattgcgc tatatggttt aggatccatt ccagatgaaa ggctctatcg
      781 aatgtttgtc aataaa
//
