LOCUS       sequence-158160-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  bacterial expression of murine TET1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Weber AR, Krawczyk C, Robertson AB, Kusnierczyk A, Vagbo CB, 
            Schuermann D, Klungland A, Schar P
  TITLE     Biochemical reconstitution of TET1-TDG-BER-dependent active DNA 
            demethylation reveals a highly coordinated mechanism.
  JOURNAL   Nat Commun. 2016 Mar 2;7:10806. doi: 10.1038/ncomms10806.
  PUBMED    26932196
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-2"; volume: "7:10806. doi"; pages: " 
            10.1038/ncomms10806"
COMMENT     Sequence Label: pCDF-mTET1
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(13..57)
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tgcgctagta gacgagtcca tgtgctggcg ttcaaatttc gcagcagcgg tttctttacc
       61 agactcgagt caatggtgat ggtgatgatg cccgggaact agatagaccc aacgattgta
      121 gggtcccgca acatgagtga gagagtatgg ggacacggta acaacattgt ctcgggttaa
      181 ggttgatgca actcgagaag gaatttggtg tgataaattg gcttcggggg atacatcagg
      241 acactcccgg tctgcgggct tttttttcgt tacttttttg catttacact taattttgtt
      301 gatatcaaaa ccatgattag gcttgtttag gcttttgtgc tggtagaata caagggacac
      361 acgaaaaggg acccctcgtt tgggggagcg caaagaggtg gtagcatgaa gctccttccg
      421 agcgcactca atgagcaccg agccgtgaat gggtgctatc gccacgccac caaaggaagg
      481 atcggcgtag atctcctcac tgtctgacca gaattcatca agttgaggca agttatcgtc
      541 ctcggggtgc tgaggctcat ccgcttcagg gtgccgctca tcttccacct gacaggaagc
      601 cagtttctga gaattgctga ggagagggag ctgctggttt gactggttag aagttagctg
      661 ctcagatgga ctagtgagag gctcagcatg aacgggagaa tcggctgtgg gaagagactg
      721 gtgaggatga gcagccacct cggcaggctg caccgttcca gtacattccc cagcagctgc
      781 attagcacca ctgtgtgctc cagaaggcat tgtgcagtgg agaatactac ttgttt
//
