LOCUS       sequence-158353-         737 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pETconNK Empty Backbone.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 737)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 737)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pETconNK
FEATURES             Location/Qualifiers
     source          1..737
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(294..323)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(439..465)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(466..477)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 cattcatctt tttttagcta ctaataggat aaattatagg aatttataac aaattgaaat
       61 atggcaggca gcaaaattaa aattgtctta gttttttagt gtatagaagt gaatagctat
      121 ataaagtatg tgtaaagttg gtaacggaac gaaaaataga aaaggatatt acatgggaaa
      181 acatgttgtt tacggagaaa tgaaaagtat attgtatttt gtacgagcta aaagtacagt
      241 gggaacaaag tcgattttgt tacatctaca ctgttgttat cagatctcta ttacaagtcc
      301 tcttcagaaa taagcttttg ttcggatccg cccccctcga gataaggcgc cttaattaag
      361 catgcttcac atatggctag ccgaccctcc gcctccgcta ccgcctccac cagagcctcc
      421 tccaccacta gcctgcagag cgtagtctgg aacgtcgtat gggtatctac cttcaatcgt
      481 cgagctattg tccttaaaaa catactgtgt gtttatgggg ctgcctttgc tagttgttga
      541 ggggtgagaa ccgcaattac tgacaaacgt tactgatttg taatattcaa aaactccttg
      601 cattgccttc ccgttggcca aaatagtagt cgttgacaaa gagtacggcg tcgattctaa
      661 agttggtgag gggatttgct cgcatatagt tgtcagttcc tgtgctaaaa ctgaagcaat
      721 aacagaaaat attgaaa
//
