LOCUS       sequence-158377-         943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1b - Step1 vector b (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 943)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1b
FEATURES             Location/Qualifiers
     source          1..943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(369..402)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(448..467)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    492..540
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
ORIGIN
        1 cggtgtgcgt cacccggcaa ccttgggcag cagcgaagtc gaggcatttc tgtcctggct
       61 ggcgaacgag cgcaaggttt cggtctccac gcatcgtcag gcattggcgg ccttgctgtt
      121 cttctacggc aaggtgctgt gcacggatct gcccttgctt caggagatcg gtagacctcg
      181 gccgtcgcgg cgcttgccgg tggtgctgac cccggatgaa gtggttcgca tcctcggttt
      241 tctggaaggc gagcatcgtt tgttcgccca ggactctagc tatagttcta gtggttggct
      301 acagctttgt ttgtactatc aacaggttga actgctgatc aacagatcct ctacgcggcc
      361 gcggtaccat aacttcgtat agcatacatt atacgaagtt atctgggttt aaactatatc
      421 tcaatcgggg tttttatcga ggctaactga aacacggaag gagacaatac cggaaggaac
      481 ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg tcgtttgttc
      541 ataaacgcgg ggttcggtcc cagggctggc actctgtcaa gtgaacgctc tatggtctaa
      601 agatttaaat ctatatctca atcggggtta cgtcgatacc ccaccgagac cccattggga
      661 ccaatacgcc cgcgtttctt ccttttcccc accccaaccc ccaagttcgg gtgaaggccc
      721 agggctcgca gccaacgtcg gggcggcaag ccctgccata gccactacgg gttaattaaa
      781 aactggatac tattgcacgt ttaaacagga gatccgaacc agataagtga aatctagttc
      841 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
      901 ctattttgtc actcttccct aaataatcct taaaaactcc att
//
