LOCUS       sequence-158388-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pBIG1e - Step1 vector e (biGBac system for expression of protein 
            complexes in insect cells).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Weissmann F, Petzold G, VanderLinden R, Huis In 't Veld PJ, Brown 
            NG, Lampert F, Westermann S, Stark H, Schulman BA, Peters JM
  TITLE     biGBac enables rapid gene assembly for the expression of large 
            multisubunit protein complexes.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 May 10;113(19):E2564-9. doi: 
            10.1073/pnas.1604935113. Epub 2016 Apr 25.
  PUBMED    27114506
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1604935113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-05-10- 10"; volume: "113"; issue: "19"; pages: "E2564-9"
COMMENT     Sequence Label: pBIG1e
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    359..407
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     432..451
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     protein_bind    497..530
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        join(926..931,932..948,949..954)
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
ORIGIN
        1 gggaactggg tgtagcgtcg taagctaata cgaaaattaa aaatgacaaa atagtttgga
       61 actagatttc acttatctgg ttcggatctc ctgtttaaac caagtcaatg tcagtgtttt
      121 taattaaccc gtagtggcta tggcagggct tgccgccccg acgttggctg cgagccctgg
      181 gccttcaccc gaacttgggg gttggggtgg ggaaaaggaa gaaacgcggg cgtattggtc
      241 ccaatggggt ctcggtgggg tatcgacgta accccgattg agatatagat ttaaatcttt
      301 agaccataga gcgttcactt gacagagtgc cagccctggg accgaacccc gcgtttatga
      361 acaaacgacc caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt
      421 tccttccggt attgtctcct tccgtgtttc agttagcctc gataaaaact aagctatgtg
      481 aaccgtttaa acccagataa cttcgtataa tgtatgctat acgaagttat ggtaccgcgg
      541 ccgcgtagag gatctgttga tcagcagttc aacctgttga tagtacaaac aaagctgtag
      601 ccaaccacta gaactatagc tagagtcctg ggcgaacaaa cgatgctcgc cttccagaaa
      661 accgaggatg cgaaccactt catccggggt cagcaccacc ggcaagcgcc gcgacggccg
      721 aggtctaccg atctcctgaa gcaagggcag atccgtgcac agcaccttgc cgtagaagaa
      781 cagcaaggcc gccaatgcct gacgatgcgt ggagaccgaa accttgcgct cgttcgccag
      841 ccaggacaga aatgcctcga cttcgctgct gcccaaggtt gccgggtgac gcacaccgtg
      901 gaaacggatg aaggcacaaa cccagttgac ataagcctgt tcggttcgta aactgtaatg
      961 caagtagcgt atgc
//
