LOCUS       sequence-158408-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pSALECT plasmid with levoglucosan kinase fused to TEM-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Klesmith JR, Bacik JP, Wrenbeck EE, Michalczyk R, Whitehead TA
  TITLE     Trade-offs between enzyme fitness and solubility illuminated by deep
            mutational scanning.
  JOURNAL   Proc Natl Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114.
  PUBMED    28196882
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2017 Feb 14. pii: 201614437. doi: 
            10.1073/pnas.1614437114."
COMMENT     Sequence Label: pSALECTNK-LGK/csTEM1
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cacaggatct cgacatgaac aataacgatc tctttcaggc atcacgtcgg cgttttctgg
       61 cacaactcgg cggcttaacc gtcgccggga tgctggggcc gtcattgcta acgccgcgac
      121 gtgcgactgc gcatatgccg attgcgacct caacgggtga taatgttctg gactttacgg
      181 ttctgggcct gaatagcggt acgagtatgg atggtattga ctgcgcactg tgtcatttct
      241 atcagaaaac cccggatgct ccgatggaat ttgaactgct ggaatacggc gaagttccgc
      301 tggcgcagcc gattaaacaa cgtgtcatgc gcatgatcct ggaagatacc acgagcccgt
      361 ctgaactgtc agaagtcaac gtgattctgg gtgaacattt tgcggatgcc gtccgtcagt
      421 tcgcggccga acgcaatgtg gatctgtcaa ccattgacgc aatcgcttcg cacggccaga
      481 cgatttggct gctgagtatg ccggaagaag gtcaagtgaa atccgccctg accatggcag
      541 aaggcgctat cctggcgagt cgtacgggta ttacctccat cacggatttc cgtatttccg
      601 accaggcagc tggtcgtcaa ggtgcaccgc tgatcgcatt tttcgatgct ctgctgctgc
      661 atcacccgac caaactgcgc gcgtgccaga acattggcgg tatcgccaat gtgtgtttta
      721 ttccgccgga tgttgacggc cgtcgcaccg atgaatatta cgattttgac acgggtccgg
      781 gcaacgtgtt catcgacgca gtggttcgtc attttaccaa tggtgaacag gaatatgata
      841 aagacggtgc tatgggcaaa cgcggtaa
//
