LOCUS       sequence-158434-         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-PmCDA1-UGI and gRNA(HPRT) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Nishida K, Arazoe T, Yachie N, Banno S, Kakimoto M, Tabata M, 
            Mochizuki M, Miyabe A, Araki M, Hara KY, Shimatani Z, Kondo A
  TITLE     Targeted nucleotide editing using hybrid prokaryotic and vertebrate 
            adaptive immune systems.
  JOURNAL   Science. 2016 Aug 4. pii: aaf8729.
  PUBMED    27492474
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2016 Aug 4. pii: aaf8729."
COMMENT     Sequence Label: pcDNA3.1_pCMV-dCas-PmCDA1-ugi pH1-gRNA(HPRT)
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     54..73
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        54..72
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             101..121
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
       61 actcactata gggagaccca agctggctag cgaaatggca ccgaagaaga agcgtaaagt
      121 cggaatccac ggagttcctg cggcaatgga caagaagtac tccattgggc tcgctatcgg
      181 cacaaacagc gtcggttggg ccgtcattac ggacgagtac aaggtgccga gcaaaaaatt
      241 caaagttctg ggcaataccg atcgccacag cataaagaag aacctcattg gcgccctcct
      301 gttcgactcc ggggagacgg ccgaagccac gcggctcaaa agaacagcac ggcgcagata
      361 tacccgcaga aagaatcgga tctgctacct gcaggagatc tttagtaatg agatggctaa
      421 ggtggatgac tctttcttcc ataggctgga ggagtccttt ttggtggagg aggataaaaa
      481 gcacgagcgc cacccaatct ttggcaatat cgtggacgag gtggcgtacc atgaaaagta
      541 cccaaccata tatcatctga ggaagaagct tgtagacagt actgataagg ctgacttgcg
      601 gttgatctat ctcgcgctgg cgcatatgat caaatttcgg ggacacttcc tcatcgaggg
      661 ggacctgaac ccagacaaca gcgatgtcga caaactcttt atccaactgg ttcagactta
      721 caatcagctt ttcgaagaga acccgatcaa cgcatccgga gttgacgcca aagcaatcct
      781 gagcgctagg ctgtccaaat cccggcggct cgaaaacctc atcgcacagc tccctgggga
      841 gaagaagaac ggcctgtttg gtaatcttat cgcc
//
