LOCUS       sequence-158444-         348 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTIM in vitro transcription backbone plasmid with two multiple 
            cloning sites and two Rho-independent transcriptional terminators..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Tapscott T, Kim JS, Crawford MA, Fitzsimmons L, Liu L, Jones-Carson 
            J, Vazquez-Torres A
  TITLE     Guanosine tetraphosphate relieves the negative regulation of 
            Salmonella pathogenicity island-2 gene transcription exerted by the 
            AT-rich ssrA discriminator region.
  JOURNAL   Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.1038/s41598-018-27780-9.
  PUBMED    29930310
REFERENCE   2  (bases 1 to 348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-21"; volume: "8(1):9465. doi"; pages: " 
            10.1038/s41598-018-27780-9"
COMMENT     Sequence Label: pTIM
FEATURES             Location/Qualifiers
     source          1..348
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      211..297
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gctgatcgtg gaccggaagg tgagccagtg agttgattgc agtccagtta cgctggagtc
       61 tgaggctcgt cctgaatgat atgcgaccgc cggagggttg cgtttgagac gggcgacaga
      121 tcgacactgc tcgatccgct cgcaccatcg aattcggtac caagcttgct agcccatggc
      181 tgcaggcgag agtagggaac tgccaggcat caaataaaac gaaaggctca gtcgaaagac
      241 tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tcctgagtag gacaaatccg
      301 ccgggagcgg atttgaacgt tgcgaagcaa cggcccggag ggtggcgg
//
