LOCUS       sequence-158445-         288 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pTIM in vitro transcription backbone plasmid with two multiple 
            cloning sites and two Rho-independent transcriptional terminators..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 288)
  AUTHORS   Tapscott T, Kim JS, Crawford MA, Fitzsimmons L, Liu L, Jones-Carson 
            J, Vazquez-Torres A
  TITLE     Guanosine tetraphosphate relieves the negative regulation of 
            Salmonella pathogenicity island-2 gene transcription exerted by the 
            AT-rich ssrA discriminator region.
  JOURNAL   Sci Rep. 2018 Jun 21;8(1):9465. doi: 10.1038/s41598-018-27780-9.
  PUBMED    29930310
REFERENCE   2  (bases 1 to 288)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2018-06-21"; volume: "8(1):9465. doi"; pages: " 
            10.1038/s41598-018-27780-9"
COMMENT     Sequence Label: pTIM
FEATURES             Location/Qualifiers
     source          1..288
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      125..152
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ttgtcggtga acgctctcct gagtaggaca aatccgccgg gagcggattt gaacgttgcg
       61 aagcaacggc ccggagggtg gcgggcagga cgcccgccat aaactgccag gcatcaaatt
      121 aagcagaagg ccatcctgac ggatggcctt tttgcgtttc tacaaactct tcctgtcgtc
      181 gactctagag agctcatgca tggatccgtt aactctgggc ggttctgata acgagtaatc
      241 gttaatccgc aaataacgta aaaacccgct tcggcgggtt tttttatg
//
