LOCUS       sequence-158490-        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  fission yeast epitope tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   Amelina H, Moiseeva V, Collopy LC, Pearson SR, Armstrong CA, Tomita 
            K
  TITLE     Sequential and counter-selectable cassettes for fission yeast.
  JOURNAL   BMC Biotechnol. 2016 Nov 8;16(1):76.
  PUBMED    27825338
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-11-8"; volume: "16(1)"; pages: "76"
COMMENT     Sequence Label: pNX3s2-PK3
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             48..89
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             93..134
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             135..176
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     terminator      206..393
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(261..285)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     polyA_signal    413..494
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     438..457
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(508..882)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVCVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(901..948)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
ORIGIN
        1 cttcgtacgc tgcaggtcga cggatccccg ggttaattaa cgctagcggt aagcctattc
       61 ctaaccctct tttgggtctc gattctacag gtggaaaacc aatccccaac ccactcctcg
      121 gccttgactc aactggcaaa cccataccaa atcctttact tggattagat agtacctcta
      181 gatgaggcgc gccacttcta aataagcgaa tttcttatga tttatgattt ttattattaa
      241 ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg
      301 aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagta
      361 tgaggtcgct cttattgacc acacctctac cggcagatcc gctaggagat ctgcagtgaa
      421 aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct
      481 gcaataaaca agtttcgagg tcgagtgtca gtcctgctcc tcggccacga agtgcacgca
      541 gttgccggcc gggtcgcgca gggcgaactc ccgcccccac ggctgctcgc cgatctcggt
      601 catggccggc ccggaggcgt cccggaagtt cgtggacacg acctccgacc actcggcgta
      661 cagctcgtcc aggccgcgca cacacaccca ggccagggtg ttgtccggca ccacctggtc
      721 ctggaccgcg ctgatgaaca gggtcacgtc gtcccggacc acaccggcga agtcgtcctc
      781 cacgaagtcc cgggagaacc cgagccggtc ggtccagaac tcgaccgctc cggcgacgtc
      841 gcgcgcggtg agcaccggaa cggcactggt caacttggcc atggtttagt tcctcacctt
      901 gtcgtattat actatgccga tatactatgc cgatgattaa ttgtcaacac ggtccgttcc
      961 aatgcaccgt tcccggccgc ggaggctgga tcggtcccgg tgtcttctat ggaggtcaa
//
