LOCUS       sequence-158502-         924 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  All-in-one doxycycline inducible lentiviral vector for expression of
            one or two genes using the P2A self-cleaving peptide..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 924)
  AUTHORS   Barger CJ, Branick C, Chee L, Karpf AR
  TITLE     Pan-Cancer Analyses Reveal Genomic Features of FOXM1 Overexpression 
            in Cancer.
  JOURNAL   Cancers (Basel). 2019 Feb 21;11(2). pii: cancers11020251. doi: 
            10.3390/cancers11020251.
  PUBMED    30795624
REFERENCE   2  (bases 1 to 924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancers 
            (Basel)."; date: "2019-02-21"; volume: "11(2). pii: cancers11020251.
            doi"; pages: " 10.3390/cancers11020251"
COMMENT     Sequence Label: pCW57-MCS1-P2A-MCS2 (Hygro)
FEATURES             Location/Qualifiers
     source          1..924
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(51..59,60..95,96..98,99..134,135..137,
                     138..173,174..179,180..797))
                     /codon_start=1
                     /product="improved tetracycline-controlled transactivator"
                     /label=rtTA-Advanced
                     /note="In the Tet-On(R) system, rtTA-Advanced binds to the 
                     Tet-responsive element and stimulates transcription only in
                     the presence of tetracycline or doxycycline."
                     /translation="MSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     CDS             complement(798..851)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 aaggagcaac atagttaaga atacgatatc ttggatcgat ccttacttag ttacccgggg
       61 agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag gtcaaagtcg
      121 tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc ggtcggcccg
      181 ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag gccgaaaagg
      241 aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtctcag aagtgggggc
      301 atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc ctgttcctcc
      361 aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc gttctccagg
      421 gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta ctgtttctct
      481 gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag agcacagcgg
      541 tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag ggggcagaag
      601 tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc ccgcttgttc
      661 ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag tttccttgtc
      721 gtcaggcctt cgataccgac accattgagt aattccagag ctccgtttat gactttgctc
      781 ttgtccagtc tagacattgg accagggttt tcttcaacat caccacaagt gaggagagaa
      841 cctctacctt ctccgcttcc ttcgaattcc tttgccctcg gacgagtgct ggggcgtcgg
      901 tttccactat cggcgagtac ttct
//
