LOCUS       sequence-158552-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  CRISPaint gene tagging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCRISPaint-SunTag-PuroR
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             61..117
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             136..189
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 tgatagctag cgggccagta cccaaaaagc ggggggtctg gtggcagtgg agggggatcc
       61 gaggagctgc tgagcaagaa ctaccacctg gagaacgagg tggccagact gaagaagggc
      121 agcggctccg gaggagaggg cagaggaagt ctgctaacat gcggtgacgt cgaggagaat
      181 cctggcccaa tgactgaata caagcctact gtcaggttgg ctacaagaga cgacgttcct
      241 agagccgtga gaactctggc tgcagccttc gccgactacc ccgccacgag acacaccgtt
      301 gacccagatc ggcatattga gagagtgact gaactgcagg agctgtttct tacaagagtt
      361 ggcctcgaca taggcaaggt gtgggtggcg gacgacggcg ccgccgtggc cgtctggacc
      421 actcccgaat cagttgaggc tggcgccgta ttcgctgaga tcggcccgag aatggctgag
      481 ctcagcggga gtaggctcgc ggcacagcag caaatggagg gactgctggc accacacagg
      541 cccaaagaac ccgcctggtt cctggcaacc gtcggtgtat ctcccgatca tcaggggaaa
      601 ggtctgggct ctgccgtagt gctccctggc gtggaggcag ctgagagagc aggagtacct
      661 gccttcttgg agacctccgc tccaaggaat cttcccttct atgaacggtt gggcttcacc
      721 gtgacagccg acgtggaagt ccccgaaggc ccccgcactt ggtgcatgac gaggaa
//
