LOCUS       sequence-158566-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9, mCherry, and sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry empty
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             264..317
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag gacggcggcc
       61 actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag ctgcccggcg
      121 cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac accatcgtgg
      181 aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag ctgtacaagg
      241 tcgctaccat cgagggatcc ggagagggca gaggaagtct gctaacatgc ggtgacgtcg
      301 aggagaatcc tggcccaatg gacaagaagt acagcatcgg cctggacatc ggcaccaaca
      361 gcgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaag ttcaaggtgc
      421 tgggcaacac cgaccgccac agcatcaaga agaacctgat cggcgccctg ctgttcgaca
      481 gcggcgagac cgccgaggcc acccgcctga agcgcaccgc ccgccgccgc tacacccgcc
      541 gcaagaaccg catctgctac ctgcaggaga tcttcagcaa cgagatggcc aaggtggacg
      601 acagcttctt ccaccgcctg gaggagagct tcctggtgga ggaggacaag aagcacgagc
      661 gccaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag taccccacca
      721 tctaccacct gcgcaagaag ctggtggaca gcaccgacaa ggccgacctg cgcctgatct
      781 a
//
