LOCUS       sequence-158567-         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9, mCherry, and sgRNA expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Schmid-Burgk JL, Honing K, Ebert TS, Hornung V
  TITLE     CRISPaint allows modular base-specific gene tagging using a 
            ligase-4-dependent mechanism.
  JOURNAL   Nat Commun. 2016 Jul 28;7:12338. doi: 10.1038/ncomms12338.
  PUBMED    27465542
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-07-28"; volume: "7:12338. doi"; pages: " 
            10.1038/ncomms12338"
COMMENT     Sequence Label: pCAS9-mCherry empty
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             285..305
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agcgcgtgat cctggccgac gccaacctgg acaaggtgct gagcgcctac aacaagcacc
       61 gcgacaagcc catccgcgag caggccgaga acatcatcca cctgttcacc ctgaccaacc
      121 tgggcgcccc cgccgccttc aagtacttcg acaccaccat cgaccgcaag cgctacacca
      181 gcaccaagga ggtgctggac gccaccctga tccaccagag catcaccggc ctgtacgaga
      241 cccgcatcga cctgagccag ctgggcggcg acagccgcgc cgaccccaag aagaagcgca
      301 aggtgtgagc ggccgcatcg ataaaataaa agattttatt tagtctccag aaaaaggggg
      361 gaatgaaaga ccccacctgt aggtttggca agctagctta agtaacgcca ttttgcaagg
      421 catggaaaaa tacataactg agaatagaga agttcagatc aaggtcagga acagatggaa
      481 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc
      541 caagaacaga tggaacagct gaatatgggc caaacaggat atctgtggta agcagttcct
      601 gccccggctc agggccaaga acagatggtc cccagatgcg gtccagccct cagcagtttc
      661 tagagaacca tcagatgttt ccagggtgcc ccaaggacct gaaatgaccc tgtgccttat
      721 ttgaactaac caatcagttc gcttctcgct tctgttcgcg cgcttctgct ccccgagctc
      781 aataaaagag cccacaaccc ctcactcggg gcgccagtcc tccgattgac tgagtcgccc
      841 gggtacccgt gtatccaata aaccctcttg cagttgcatc cgacttg
//
