LOCUS       sequence-158572-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a 
            Thosea asigna virus peptide (2A), and membrane-tethered mCherry 
            protein (membCherry); flanked by Tol2 sequences.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Trinh LA, Chong-Morrison V, Gavriouchkina D, Hochgreb-Hagele T, 
            Senanayake U, Fraser SE, Sauka-Spengler T
  TITLE     Biotagging of Specific Cell Populations in Zebrafish Reveals Gene 
            Regulatory Logic Encoded in the Nuclear Transcriptome.
  JOURNAL   Cell Rep. 2017 Apr 11;19(2):425-440. doi: 
            10.1016/j.celrep.2017.03.045.
  PUBMED    28402863
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.03"; journalName: "Cell Rep"; date: 
            "2017-04-11- 11"; volume: "19"; issue: "2"; pages: "425-440"
COMMENT     Sequence Label: pMTB2-cytoBirA-2A-mCherry_Ras
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    join(6..17,18..205)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 agaaccagag gtgtaaagta cttgagtaat tttacttgat tactgtactt aagtattatt
       61 tttggggatt tttactttac ttgagtacaa ttaaaaatca atacttttac ttttacttaa
      121 ttacattttt ttagaaaaaa aagtactttt tactccttac aattttattt acagtcaaaa
      181 agtacttatt ttttggagat cacttgggcc cggctcgagg gcctagtcat tacctatact
      241 caacgtttac tctgagaaac ttccatattt tagaggtaaa atgctcagaa ttgtaaaaag
      301 tcttgtcaaa atacttggaa tttgccaaaa acaaaataaa agtcacatca cctcattgtc
      361 cttggtatca gcaagcactt ttaacacaat cagctgtttt tgtttataac tcccactgaa
      421 ggccatgtgc attactccca agttacacag taaaaagtca catgtagttt atcatgatca
      481 ctgctgctca agcacagctt tatctgttat tcagtgtggc aatatctgaa atgtgtgcaa
      541 aatattattt attgtagctg atcaccaaca ctttcagtgt tttttttttt ctacttttaa
      601 tgagaattat gttcttagga tttatcctta taaacctctt taaaaagaaa tgttatgtct
      661 gcatagacac gacatgaaat tatgaacaga aaatttaact atttaagatc aagacttgac
      721 atataacatt tctaatgaac attaaacctg aggtaggccc acttgcaaaa atgtaactgc
      781 tatcactcac tttatacatc attgtgtaac ttgtacacac aaaaatagtg aagcaatgtg
      841 gcatggaatg caggtcccat tcattcaaag ttattaaagc actttcttaa agtgcattac
      901 tactgagcta agaccagcta gttgctcata caacatacta caagtag
//
