LOCUS       sequence-158606-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  No anti-scaffold control with 3xFLAG tags for Western blots. 
            Contains pBAD-CusR (response regulator) and pCusR-GFP(3xFLAG)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Hsiao V, de los Santos EL, Whitaker WR, Dueber JE, Murray RM
  TITLE     Design and implementation of a biomolecular concentration tracker.
  JOURNAL   ACS Synth Biol. 2015 Feb 20;4(2):150-61. doi: 10.1021/sb500024b. 
            Epub 2014 May 15.
  PUBMED    24847683
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500024b";
            journalName: "ACS Synth Biol"; date: "2015-02-20- 20"; volume: "4"; 
            issue: "2"; pages: "150-61"
COMMENT     Sequence Label: pVH016
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(7..24)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(40..69)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(89..154)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             complement(155..868)
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
ORIGIN
        1 aactcaatga tgatgatgat gatggtcgac ggcgctattc agatcctctt ctgagatgag
       61 tttttgttcg ggcccaagct tctcactact tgtcatcgtc atccttgtag tcgatgtcat
      121 gatccttgta atcaccgtca tggtccttgt agtctttgta cagttcatcc ataccatgcg
      181 tgatgcccgc tgcggttacg aactccagca gaaccatatg atcgcgtttc tcgttcggat
      241 ctttagacag aacgctttgc gtgctcagat agtgattgtc tggcagcaga acaggaccat
      301 caccgattgg agtgttttgc tggtagtgat cagccagctg cacgctgcca tcctccacgt
      361 tgtggcgaat tttaaaattc gctttaatgc catttttttg tttatcggcg gtgatgtaaa
      421 cattgtggct gttaaaattg tattccagct tatggcccag gatattgccg tcttctttaa
      481 agtcaatgcc tttcagctca atgcggttta ccagggtatc gccttcaaat ttcacttccg
      541 cacgcgtttt gtacgtgccg tcatccttaa aggaaatcgt gcgttcctgc acatagcctt
      601 ccggcatggc ggacttgaag aagtcatgct gcttcatatg gtccggataa cgagcaaagc
      661 actgaacacc ataagtcagc gtcgttacca gagtcggcca aggaaccggc agtttaccag
      721 tagtacagat gaacttcagc gtcagtttac cattagttgc gtcaccttca ccctcgccac
      781 gcacggaaaa cttatgaccg ttgacatcac catccagttc caccagaata gggacgacac
      841 cagtgaacag ctcttcgcct ttacgcatct aatcctcctg cgcccgtttc ttacgaatgt
      901 atgagctcat aa
//
