LOCUS       sequence-158613-        5111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Used for abrB0310(CAC0310) overexpression in Clostridium 
            acetobutylicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5111)
  AUTHORS   Xue Q, Yang Y, Chen J, Chen L, Yang S, Jiang W, Gu Y
  TITLE     Roles of three AbrBs in regulating two-phase Clostridium 
            acetobutylicum fermentation.
  JOURNAL   Appl Microbiol Biotechnol. 2016 Jun 8.
  PUBMED    27276910
REFERENCE   2  (bases 1 to 5111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2016 Jun 8."
COMMENT     Sequence Label: pIMP1-Pthl-AbrB0310
FEATURES             Location/Qualifiers
     source          1..5111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(452..469)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(452..468)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(461..483)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(677..696)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     796..818
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(856..874)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        942..1046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1047..1115,1116..1907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1265..1284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2078..2666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2567..2586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2820..2837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2954..2975
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2990..2995,2996..3013,3014..3020)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3028..3044
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3033..3055
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3052..3068
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3052..3068
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agcttggctg cagtttttaa caaaatatat tgataaaaat aataatagtg ggtataatta
       61 agttgttaga gaaaacgtat aaattaggga taaactatgg aacttatgaa atagattgaa
      121 atggtttatc tgttaccccg tatcaaaatt taggaggtta gttagagtcg actctagaat
      181 gaaatcaaca ggtgtagtta gaagagtaga tgaattaggt agaattgtaa tacctataga
      241 gttaagaaga actttaaata tagcagaaaa agacgcttta gaaatttatg tagatggtga
      301 gcaaatcatt ttaaagaaat atgaacctgc atgtatattc tgcggagatg caagcgatgt
      361 tatcaactat agaggaaaaa acatatgtaa gcattgtctt gaagaattaa aagaaggtag
      421 ataaggatcc ccgggtaccg agctcgaatt cactggccgt cgttttacaa cgtcgtgact
      481 gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct
      541 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      601 gcgaatggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
      661 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
      721 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
      781 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
      841 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
      901 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
      961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     1801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     1861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2221 ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     2761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     2821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     2881 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2941 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3001 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     3061 gctatgacca tgattacgcc aagctttggc taacacacac gccattccaa ccaatagttt
     3121 tctcggcata aagccatgct ctgacgctta aatgcactaa tgccttaaaa aaacattaaa
     3181 gtctaacaca ctagacttat ttacttcgta attaagtcgt taaaccgtgt gctctacgac
     3241 caaaagtata aaacctttaa gaactttctt ttttcttgta aaaaaagaaa ctagataaat
     3301 ctctcatatc ttttattcaa taatcgcatc agattgcagt ataaatttaa cgatcactca
     3361 tcatgttcat atttatcaga gctcgtgcta taattatact aattttataa ggaggaaaaa
     3421 ataaagaggg ttataatgaa cgagaaaaat ataaaacaca gtcaaaactt tattacttca
     3481 aaacataata tagataaaat aatgacaaat ataagattaa atgaacatga taatatcttt
     3541 gaaatcggct caggaaaagg gcattttacc cttgaattag tacagaggtg taatttcgta
     3601 actgccattg aaatagacca taaattatgc aaaactacag aaaataaact tgttgatcac
     3661 gataatttcc aagttttaaa caaggatata ttgcagttta aatttcctaa aaaccaatcc
     3721 tataaaatat ttggtaatat accttataac ataagtacgg atataatacg caaaattgtt
     3781 tttgatagta tagctgatga gatttattta atcgtggaat acgggtttgc taaaagatta
     3841 ttaaatacaa aacgctcatt ggcattattt ttaatggcag aagttgatat ttctatatta
     3901 agtatggttc caagagaata ttttcatcct aaacctaaag tgaatagctc acttatcaga
     3961 ttaaatagaa aaaaatcaag aatatcacac aaagataaac agaagtataa ttatttcgtt
     4021 atgaaatggg ttaacaaaga atacaagaaa atatttacaa aaaatcaatt taacaattcc
     4081 ttaaaacatg caggaattga cgatttaaac aatattagct ttgaacaatt cttatctctt
     4141 ttcaatagct ataaattatt taataagtaa gttaagggat gcataaactg catcccttaa
     4201 cttgtttttc gtgtacctat tttttgtgaa tcgattatgt cttttgcgca ttcacttctt
     4261 ttctatataa atatgagcga agcgaataag cgtcggaaaa gcagcaaaaa gtttcctttt
     4321 tgctgttgga gcatgggggt tcagggggtg cagtatctga cgtcaatgcc gagcgaaagc
     4381 gagccgaagg gtagcattta cgttagataa ccccctgata tgctccgacg ctttatatag
     4441 aaaagaagat tcaactaggt aaaatcttaa tataggttga gatgataagg tttataagga
     4501 atttgtttgt tctaattttt cactcatttt gttctaattt cttttaacaa atgttctttt
     4561 ttttttagaa cagttatgat atagttagaa tagtttaaaa taaggagtga gaaaaagatg
     4621 aaagaaagat atggaacagt ctataaaggc tctcagaggc tcatagacga agaaagtgga
     4681 gaagtcatag aggtagacaa gttataccgt aaacaaacgt ctggtaactt cgtaaaggca
     4741 tatatagtgc aattaataag tatgttagat atgattggcg gaaaaaaact taaaatcgtt
     4801 aactatatcc tagataatgt ccacttaagt aacaatacaa tgatagctac aacaagagaa
     4861 atagcaaaag ctacaggaac aagtctacaa acagtaataa caacacttaa aatcttagaa
     4921 gaaggaaata ttataaaaag aaaaactgga gtattaatgt taaaccctga actactaatg
     4981 agaggcgacg accaaaaaca aaaatacctc ttactcgaat ttgggaactt tgagcaagag
     5041 gcaaatgaaa tagattgacc tcccaataac accacgtagt tattgggagg tcaatctatg
     5101 aaatgcgatt a
//
