LOCUS       sequence-158615-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used for abrB1941(CAC1941) overexpression in Clostridium 
            acetobutylicum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  AUTHORS   Xue Q, Yang Y, Chen J, Chen L, Yang S, Jiang W, Gu Y
  TITLE     Roles of three AbrBs in regulating two-phase Clostridium 
            acetobutylicum fermentation.
  JOURNAL   Appl Microbiol Biotechnol. 2016 Jun 8.
  PUBMED    27276910
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Microbiol Biotechnol. 2016 Jun 8."
COMMENT     Sequence Label: pIMP1-Pthl-AbrB1941
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..113
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     105..122
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     106..122
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     RBS             152..160
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga tgtgctgcaa
       61 ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa acgacggcca
      121 gtgaattcga gctcggtacc cggggatccc tacctcctta attctttcaa gcacttctta
      181 catatcttct ttcctttata atttattacg tcgcttgctt caccgcagaa cacacatgct
      241 ggattatatt tctttaagac tatctgctca ccatctacat aaatttctaa agcgtccttt
      301 tctgctatat taaatgttct tctcaattct ttaggtatta ctacccttcc caagctatcc
      361 aattttctta ctattcctgt tgatttcatt ctagagtcga ctctaactaa cctcctaaat
      421 tttgatacgg ggtaacagat aaaccatttc aatctatttc ataagttcca tagtttatcc
      481 ctaatttata cgttttctct aacaacttaa ttatacccac tattattatt tttatcaata
      541 tattttgtta aaaactgcag ccaagcttaa tcgcatttca tagattgacc tcccaataac
      601 tacgtggtgt tattgggagg tcaatctatt tcatttgcct cttgctcaaa gttcccaaat
      661 tcgagtaaga ggtatttttg tttttggtcg tcgcctctca ttagtagttc agggtttaac
      721 attaatactc cagtttttct ttttataata tttccttctt ctaagatttt aagtgttgtt
      781 attactgttt gtagacttgt tcctgtagct tttgctattt ctcttgttgt agctatcatt
      841 gtattgttac ttaagtggac attatctagg atatagttaa cgattttaag tttttt
//
