LOCUS       sequence-158632-        5622 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5622)
  AUTHORS   Snider L, Asawachaicharn A, Tyler AE, Geng LN, Petek LM, Maves L, 
            Miller DG, Lemmers RJ, Winokur ST, Tawil R, van der Maarel SM, 
            Filippova GN, Tapscott SJ
  TITLE     RNA transcripts, miRNA-sized fragments and proteins produced from 
            D4Z4 units: new candidates for the pathophysiology of 
            facioscapulohumeral dystrophy.
  JOURNAL   Hum Mol Genet. 2009 Jul 1;18(13):2414-30. doi: 10.1093/hmg/ddp180. 
            Epub 2009 Apr 9.
  PUBMED    19359275
REFERENCE   2  (bases 1 to 5622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddp180"; journalName: "Hum Mol Genet"; date: 
            "2009-07-1- 1"; volume: "18"; issue: "13"; pages: "2414-30"
COMMENT     Sequence Label: pCS2-mkgDUX4
FEATURES             Location/Qualifiers
     source          1..5622
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(63..80)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(234..822)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(314..333)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(993..1784,1785..1853))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1616..1635
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1854..1958)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(1984..2439)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2121..2142)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2333..2352
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        2510..3494
                     /label=CMV IE94 promoter
                     /note="enhancer/promoter region of simian cytomegalovirus 
                     major immediate early transcription unit IE94"
     promoter        3529..3547
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     3529..3546
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     polyA_signal    5168..5302
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5192..5211)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5246..5265
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(5415..5435)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(5415..5433)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(5454..5470)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5454..5470)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5467..5489)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5478..5494
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5502..5508,5509..5526,5527..5532))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5547..5568
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct
       61 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac
      121 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga
      181 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat
      241 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac
      301 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct
      361 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg
      421 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg
      481 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt
      541 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg
      601 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac
      661 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga
      721 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt
      781 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt
      841 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga
      901 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc
      961 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct
     1021 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata
     1081 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca
     1141 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga
     1201 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga
     1261 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg
     1321 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga
     1381 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt
     1441 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct
     1501 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca
     1561 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat
     1621 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga
     1681 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc
     1741 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg
     1801 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc
     1861 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt
     1921 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca
     1981 cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
     2041 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
     2101 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
     2161 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
     2221 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
     2281 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
     2341 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
     2401 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc
     2461 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag tcgaccgcca
     2521 attcaatatg gcgtatatgg actcatgcca attcaatatg gtggatctgg acctgtgcca
     2581 attcaatatg gcgtatatgg actcgtgcca attcaatatg gtggatctgg accccagcca
     2641 attcaatatg gcggacttgg caccatgcca attcaatatg gcggacctgg cactgtgcca
     2701 actggggagg ggtctacttg gcacggtgcc aagtttgagg aggggtcttg gccctgtgcc
     2761 aagtccgcca tattgaattg gcatggtgcc aataatggcg gccatattgg ctatatgcca
     2821 ggatcaatat ataggcaata tccaatatgg ccctatgcca atatggctat tggccaggtt
     2881 caatactatg tattggccct atgccatata gtattccata tatgggtttt cctattgacg
     2941 tagatagccc ctcccaatgg gcggtcccat ataccatata tggggcttcc taataccgcc
     3001 catagccact cccccattga cgtcaatggt ctctatatat ggtctttcct attgacgtca
     3061 tatgggcggt cctattgacg tatatggcgc ctcccccatt gacgtcaatt acggtaaatg
     3121 gcccgcctgg ctcaatgccc attgacgtca ataggaccac ccaccattga cgtcaatggg
     3181 atggctcatt gcccattcat atccgttctc acgcccccta ttgacgtcaa tgacggtaaa
     3241 tggcccactt ggcagtacat caatatctat taatagtaac ttggcaagta cattactatt
     3301 ggaagtacgc cagggtacat tggcagtact cccattgacg tcaatggcgg taaatggccc
     3361 gcgatggctg ccaagtacat ccccattgac gtcaatgggg aggggcaatg acgcaaatgg
     3421 gcgttccatt gacgtaaatg ggcggtaggc gtgcctaatg ggaggtctat ataagcaatg
     3481 ctcgtttagg gaaccgccat tctgcctggg gacgtcggag caagcttgat ttaggtgaca
     3541 ctatagaata caagctactt gttctttttg caggatccca tcgatccagg cctcgacgcc
     3601 ctgggtccct tccggggtgg ggcgggctgt cccagggggg ctcaccgcca ttcatgaagg
     3661 ggtggagcct gcctgcctgt gggcctttac aagggcggct ggctggctgg ctggctgtcc
     3721 gggcaggcct cctggctgca cctgccgcag tgcacagtcc ggctgaggtg cacgggagcc
     3781 cgccggcctc tctctgcccg cgtccgtccg tgaaattccg gccggggctc accgcgatgg
     3841 ccctcccgac accctcggac agcaccctcc ccgcggaagc ccggggacga ggacggcgac
     3901 ggagactcgt ttggaccccg agccaaagcg aggccctgcg agcctgcttt gagcggaacc
     3961 cgtacccggg catcgccacc agagaacggc tggcccaggc catcggcatt ccggagccca
     4021 gggtccagat ttggtttcag aatgagaggt cacgccagct gaggcagcac cggcgggaat
     4081 ctcggccctg gcccgggaga cgcggcccgc cagaaggccg gcgaaagcgg accgccgtca
     4141 ccggatccca gaccgccctg ctcctccgag cctttgagaa ggatcgcttt ccaggcatcg
     4201 ccgcccggga ggagctggcc agagagacgg gcctcccgga gtccaggatt cagatctggt
     4261 ttcagaatcg aagggccagg cacccgggac agggtggcag ggcgcccgcg caggcaggcg
     4321 gcctgtgcag cgcggccccc ggcgggggtc accctgctcc ctcgtgggtc gccttcgccc
     4381 acaccggcgc gtggggaacg gggcttcccg caccccacgt gccctgcgcg cctggggctc
     4441 tcccacaggg ggctttcgtg agccaggcag cgagggccgc ccccgcgctg cagcccagcc
     4501 aggccgcgcc ggcagagggg atctcccaac ctgccccggc gcgcggggat ttcgcctacg
     4561 ccgccccggc tcctccggac ggggcgctct cccaccctca ggctcctcgc tggcctccgc
     4621 acccgggcaa aagccgggag gaccgggacc cgcagcgcga cggcctgccg ggcccctgcg
     4681 cggtggcaca gcctgggccc gctcaagcgg ggccgcaggg ccaaggggtg cttgcgccac
     4741 ccacgtccca ggggagtccg tggtggggct ggggccgggg tccccaggtc gccggggcgg
     4801 cgtgggaacc ccaagccggg gcagctccac ctccccagcc cgcgcccccg gacgcctccg
     4861 cctccgcgcg gcaggggcag atgcaaggca tcccggcgcc ctcccaggcg ctccaggagc
     4921 cggcgccctg gtctgcactc ccctgcggcc tgctgctgga tgagctcctg gcgagcccgg
     4981 agtttctgca gcaggcgcaa cctctcctag aaacggaggc cccgggggag ctggaggcct
     5041 cggaagaggc cgcctcgctg gaagcacccc tcagcgagga agaataccgg gctctgctgg
     5101 aggagcttta gaagggcgaa ttcaaggcct ctcgagcctc tagaactata gtgagtcgta
     5161 ttacgtagat ccagacatga taagatacat tgatgagttt ggacaaacca caactagaat
     5221 gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat ttgtaaccat
     5281 tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt ttcaggttca
     5341 gggggaggtg tgggaggttt tttaattcgc ggccgcggcg ccaatgcatt gggcccggta
     5401 cccagctttt gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag
     5461 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccgggagc
     5521 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
     5581 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tg
//
