LOCUS       sequence-158798-         842 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP with additional bases 5' end like pCas34, but 
            from pJ23119 in the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 842)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas45
FEATURES             Location/Qualifiers
     source          1..842
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 aattcttcac acttttaacg gctgtctcca cagtgttcct gactcctatt tacggagcgg
       61 ctcaaacgat gcaagatcaa agtaatacga tgaataatga aaattcattg gggaatagtg
      121 cgactggggt agtcaaccaa accgctgtgt acaacttagg tcaaagtgcc acctacggtt
      181 ttagagggat cgtttgtccc cgcccttcct ttgtgctcag tggctctgca aatggcaata
      241 catttgatgg ggagagttca aattcctacg ctctttccgg tgccattatt gtgcccctcg
      301 gtggtcaagt gggcagcaac tgtagagatt tcagcactac cctactggaa agagaacaag
      361 ccgaattagc aaagtttcaa atccaatctt cagcggaaac agtcaaactt tgtgctgaca
      421 tgatttcttc aggcattcaa gtagatccag aggtttatcc agtactggcg cggcaatgtt
      481 ctgcagttcg gttggtttca caaggacggg cttctagcac aaatgaagcc cgacaaaatg
      541 aatcgactca caataagcta gatgatgatg tagaattgtc tgctaattac ttaaaaactg
      601 agtaaatata atattttgaa tagtgaggaa aaatatctca aaggctatat gaaaataaaa
      661 aaagagcggc taaaaaatct tcagcttaca gtgacgcctg tctttgcatt ctaaagcact
      721 aaaacacagt cgttttttta ctgactccaa gtgtttttat tactatactt agaaattcgt
      781 caaataggca tgaaattgct gtattaaaat atcctactgt atcttttagt tcacactatt
      841 tt
//
