LOCUS       sequence-158858-         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TagBFP (blue) fluorophore with stop codon for C-terminal fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Don EK, Formella I, Badrock AP, Hall TE, Morsch M, Hortle E, Hogan 
            A, Chow S, Gwee SS, Stoddart JJ, Nicholson G, Chung R, Cole NJ
  TITLE     A Tol2 Gateway-Compatible Toolbox for the Study of the Nervous 
            System and Neurodegenerative Disease.
  JOURNAL   Zebrafish. 2016 Sep 15.
  PUBMED    27631880
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Zebrafish. 
            2016 Sep 15."
COMMENT     Sequence Label: p3E-mTagBFP
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     29..48
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     150..166
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     150..166
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     171..190
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        171..189
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    227..322
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 atcgcggcct cgacgtttcc cgttgaatat ggctcataac accccttgta ttactgttta
       61 tgtaagcaga cagttttatt gttcatgatg atatattttt atcttgtgca atgtaacatc
      121 agagattttg agacacgggc cagagctgcc aggaaacagc tatgaccatg taatacgact
      181 cactataggg gatatcgcgg ccgcgttaac gctaccatgg agctccaaat aatgatttta
      241 ttttgactga tagtgacctg ttcgttgcaa caaattgata agcaatgctt ttttataatg
      301 ccaactttgt ataataaagt tgcactagtt taattaagct tgtgccccag tttgctaggg
      361 aggtcgcagt atctggccac tgccacctcg tgctgctcga cgtaggtctc gttgttggcc
      421 tccttgattc tttccagtct gtagtccaca tagtagacgc caggcatctt gaggttctta
      481 gcgggtttct tggatctata tgtggtcttg atgtttgcga tcagatggct cccgcccacg
      541 agcttcaggg ccatgtcgtt tctgccttcc aggccgccgt cagcggggta cagcgtctcg
      601 gtgaaggcct cccagccgag tgttttcttc tgcatcacag ggccgttgga tgtgaagttc
      661 acccctctga tcttgacgtt gtagatgagg cagccgtcct ggaggctggt gtcctgggta
      721 gcggtcagca cgcccccgtc ttcgtatgtg gtgactctct cccatgtgaa gccctcaggg
      781 aaggactgct tgaagaagtc ggggatgccc tgggtgtggt tgatgaaggt cttgctgccg
      841 tagaggaagc tagtagccag gatgtcgaag gcgaagggga gagggccgcc ctcgaccacc
      901 ttga
//
