LOCUS       sequence-158985-         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding murine Lmo2 downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Lmo2
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(811..835)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(908..925)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggtgg
       61 cggccatcac aagtttgtac aaaaaagcag gctccgaatt cgcccttagg atgattcgct
      121 ctctctcttt ggcgtttgaa gggagcgcgg tgactgtcct tgagcgtgga ggggcgagct
      181 cgccagcgga gcgaccgagc aagaggaggc gcaggagcgg cggcgcccga gcacccgagg
      241 gggtccgagt ccaggcagct agccagccac gcgccacaaa gggagcgccc ccgccgcccg
      301 gcacgccgcc cccctcccca atgtcctcgg ccatcgaaag gaagagcctg gacccgtctg
      361 aggaacccgt ggatgaggtg ctgcagatac ccccatccct gctgacatgt ggtggctgcc
      421 agcagaacat aggggaccgc tacttcctga aagccatcga ccagtactgg catgaggatt
      481 gcctcagctg tgacctctgt gggtgtcggc tgggagaggt ggggaggcgc ctctactaca
      541 agctgggacg gaaattgtgc aggagagact atctcaggct ttttggtcag gatggtctct
      601 gtgcatcctg tgacaagcgg atccgtgcct atgagatgac gatgcgggtg aaagacaaag
      661 tgtatcacct ggagtgtttc aaatgcgccg cctgtcagaa gcatttctgt gtaggtgaca
      721 gataccttct catcaactcc gacatagtgt gtgaacaaga catctacgag tggaccaaga
      781 tcaatgggat catctagaag ggcgaattcg acccagcttt cttgtacaaa gtggtgatcc
      841 aaccggtata gatctatggc cggccatcaa ttgatactag tcaccgactc tagagctcgc
      901 tgatcagcct cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg
//
