LOCUS       sequence-159070-         697 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express PAsfCherry2(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 697)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 697)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pcDNA3.1_PAsfCherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..697
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(246..264)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     514..533
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccatggagga
      121 ggataacatg gccatcatta aggagttcat gcgcttcaag gtgcacatgg aggggtccgt
      181 gaacggccac gtgttcgaga tcgagggcga gggcgagggc cacccctacg agggcaccca
      241 gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc ttcacctggg acatcctgtc
      301 ccctcaattc atgtacggct ccaatgccta cgtgaagcac cccgccgaca tccccgacta
      361 ctttaagctg tccttccccg agggcttcac ctgggagcgc gtgatgaaat tcgaggacgg
      421 cggcgtggtg accgtgaccc aggactcctc cctgcaggac ggtcagttca tctacaaggt
      481 gaagctgctg ggcatcaact tcccctccga cggccccgta atgcagaaga agaccatggg
      541 ctgggaggcc ctcaccgagc ggatgtaccc cgaggacggc gccctgaagg gcgaggtcaa
      601 cccgagagtg aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa
      661 ggccaagaag cccgtgcagc tgcccggcgc ctacaac
//
