LOCUS       sequence-159071-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express PAsfCherry2(1-10) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pcDNA3.1_PAsfCherry2(1-10)
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(269..288)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     538..556
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(730..749)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(731..749)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(794..845)
                     /label=CMVd2 promoter
                     /note="truncated human cytomegalovirus (CMV) immediate 
                     early promoter that yields moderately reduced expression 
                     relative to the full-length promoter"
     primer_bind     complement(823..843)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
ORIGIN
        1 ctcgagcggc cgccactgtg ctggatatct gcagaattcc accacactgg actagtggat
       61 cctcagtcct cgttgtgtga ggtgatgtcc aacttgcggt cgacgttgta ggcgccgggc
      121 agctgcacgg gcttcttggc cttgtaggtg gtcttgacct cagcgtcgta gtggccgccg
      181 tccttcagct tcactctcgg gttgacctcg cccttcaggg cgccgtcctc ggggtacatc
      241 cgctcggtga gggcctccca gcccatggtc ttcttctgca ttacggggcc gtcggagggg
      301 aagttgatgc ccagcagctt caccttgtag atgaactgac cgtcctgcag ggaggagtcc
      361 tgggtcacgg tcaccacgcc gccgtcctcg aatttcatca cgcgctccca ggtgaagccc
      421 tcggggaagg acagcttaaa gtagtcgggg atgtcggcgg ggtgcttcac gtaggcattg
      481 gagccgtaca tgaattgagg ggacaggatg tcccaggtga agggcagggg gccacccttg
      541 gtcaccttca gcttggcggt ctgggtgccc tcgtaggggt ggccctcgcc ctcgccctcg
      601 atctcgaaca cgtggccgtt cacggacccc tccatgtgca ccttgaagcg catgaactcc
      661 ttaatgatgg ccatgttatc ctcctccatg gtaccaagct taagtttaaa cgctagccag
      721 cttgggtctc cctatagtga gtcgtattaa tttcgataag ccagtaagca gtgggttctc
      781 tagttagcca gagagctctg cttatataga cctcccaccg tacacgccta ccgcccattt
      841 gcgtcaatgg ggcggagttg ttacgacatt
//
