LOCUS       sequence-159182-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of STK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: STK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             384..392
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
ORIGIN
        1 ctttcgggct ttgttagcag ccggatcagc gcgcacgcgt ggtaccggcc ggccaccagg
       61 tggcgcgccg cgatcgcgga tccgttatcc acttccaatg ttattattct tcctcttcca
      121 attctcgttg ctgttcctca tgtcttttag ctttgatctc catagcttct gtgatcaggt
      181 ctcttaatat tgatacaggt ttggcattct tgataaaagg atgctgtaaa agttgtgttg
      241 cagtagctct ctgctcagga ttcttcacca aacacttttt aacaaaatcg gtgaaatcat
      301 cggaccaaag ttctggcttt ctgaatgttg gtggtggatt tgtgggaatc ataaaaatag
      361 ccctcattgg atgtatatca gcataaggag gttttccttc agccatttct atagaagtaa
      421 tgccaaggga ccagatgtcg gccacacagt tatagcctat ttcttgaatc acctcaggag
      481 ccatccaaaa tggagttcct attacagtat tgcgttttgc cattgtatct gttaactgac
      541 cagccactcc aaaatctgcc aattttgcat gtccttctgt attgaggaga atatttccag
      601 cttttatatc tctgtgtatt tttctcataa agtgcaaata ttctagtcct ttcaatgtag
      661 atttaagaat ggttgcaatt tcatcttcta ttaatgtctt gtttcgtaat ctaattatgt
      721 ctgagacaga gccagcgcca cagtactcca taacaatcca gaggtctgta ttcttaaaat
      781 aactgccata gtactttaca acatatgggc tgtcacattg ctgcattatg gaaatttctt
      841 tgattatttc ctgaagatct gattcaacag gtacttgttt aattgcgaca acttgaccgg
      901 attccttgtg tattgcttta aatacacttc cataagaccc ttctccaagc ttctctaata
      961 catcaaaaac ttcttcaggc tgct
//
