LOCUS       sequence-159215-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop 
            codon (for native or N-terminal fusions), G12D mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.KRAS4a G12D
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(695..794)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattcgagct cggtccgagg atccgaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagtt
      121 ggcaccatga ctgaatataa acttgtggta gttggagctg atggcgtagg caagagtgcc
      181 ttgacgatac agctaattca gaatcatttt gtggacgaat atgatccaac aatagaggat
      241 tcctacagga agcaagtagt aattgatgga gaaacctgtc tcttggatat tctcgacaca
      301 gcaggtcaag aggagtacag tgcaatgagg gaccagtaca tgaggactgg ggagggcttt
      361 ctttgtgtat ttgccataaa taatactaaa tcatttgaag atattcacca ttatagagaa
      421 caaattaaaa gagttaagga ctctgaagat gtacctatgg tcctagtagg aaataaatgt
      481 gatttgcctt ccagaacagt agacacaaaa caggctcagg acttagcaag aagttatgga
      541 attcctttta ttgaaacatc agcaaagaca agacagagag tggaggatgc tttttataca
      601 ttggtgagag agatccgaca atacagattg aaaaaaatca gcaaagaaga aaagactcct
      661 ggctgtgtga aaattaaaaa atgcattata atgtaatcaa ctttcttgta caaagttggc
      721 attataggaa agcattgctt atcaatttgt tgcaacgaac aggtcactat cagtcaaaat
      781 aaaatcatta tttgcatgca agcttagatc ctctagacca gccaggacag aaatgcctcg
      841 acttcgctgc tacc
//
