LOCUS       sequence-159225-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the dehydrogenase-deficient mutant 
            DLD-H450A..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Malty RH, Aoki H, Kumar A, Phanse S, Amin S, Zhang Q, Minic Z, 
            Goebels F, Musso G, Wu Z, Abou-Tok H, Meyer M, Deineko V, Kassir S, 
            Sidhu V, Jessulat M, Scott NE, Xiong X, Vlasblom J, Prasad B, Foster
            LJ, Alberio T, Garavaglia B, Yu H, Bader GD, Nakamura K, Parkinson 
            J, Babu M
  TITLE     A Map of Human Mitochondrial Protein Interactions Linked to 
            Neurodegeneration Reveals New Mechanisms of Redox Homeostasis and 
            NF-kappaB Signaling.
  JOURNAL   Cell Syst. 2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010.
  PUBMED    29128334
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Syst. 
            2017 Nov 7. pii: S2405-4712(17)30447-7. doi: 
            10.1016/j.cels.2017.10.010."
COMMENT     Sequence Label: pPHAGE_DLD-H450A-C-TAP
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(555..579)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             611..634
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             650..676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YPYDVPDYA"
     CDS             650..676
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(844..861)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 agagctggga attgaactag atcccagagg tagaattcca gtcaatacca gatttcaaac
       61 taaaattcca aatatctatg ccattggtga tgtagttgct ggtccaatgc tggctcacaa
      121 agcagaggat gaaggcatta tctgtgttga aggaatggct ggtggtgctg tgcacattga
      181 ctacaattgt gtgccatcag tgatttacac acaccctgaa gttgcttggg ttggcaaatc
      241 agaagagcag ttgaaagaag agggtattga gtacaaagtt gggaaattcc catttgctgc
      301 taacagcaga gctaagacaa atgctgacac agatggcatg gtgaagatcc ttgggcagaa
      361 atcgacagac agagtactgg gagcacatat tcttggacca ggtgctggag aaatggtaaa
      421 tgaagctgct cttgctttgg aatatggagc atcctgtgaa gatatagcta gagtctgtgc
      481 agcacatccg accttatcag aagcttttag agaagcaaat cttgctgcgt catttggcaa
      541 atcaatcaac ttttgcccaa ctttcttgta caaagtggtt gatctagagg gcccgcggtt
      601 cgaactcgag gactacaagg acgacgatga caagtcggcc gctggaggat acccctacga
      661 cgtgcccgac tacgcctaga attccgcccc ccccccctaa cgttactggc cgaagccgct
      721 tggaataagg ccggtgtgcg tttgtctata tgttattttc caccatattg ccgtcttttg
      781 gcaatgtgag ggcccggaaa cctggccctg tcttcttgac gagcattcct aggggtcttt
      841 cccctctcgc caaaggaatg caaggtctgt tgaatgtcgt gaaggaagca gttcctctgg
      901 aagcttcttg aagacaa
//
