LOCUS       sequence-159238-        1048 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of EPHA3. Intended for 
            co-expression with YopH Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1048)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1048)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: EPHA3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1048
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             62..84
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             104..133
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             134..154
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 cggctccgga gagctcttta attaagcggc cgccctgcag gactcgagtt ctagaaataa
       61 ttttgtttaa ctttaagaag gagatataca tatgaaatct tctcaccatc accatcacca
      121 tcaccatcac catgaaaacc tgtacttcca atccaatgca acccaagctg ttcatgagtt
      181 tgccaaggaa ttggatgcca ccaacatatc cattgataaa gttgttggag caggtgaatt
      241 tggagaggtg tgcagtggtc gcttaaaact tccttcaaaa aaagagattt cagtggccat
      301 taagaccctg aaagttggct acacagaaaa gcagaggaga gacttcctgg gagaagcaag
      361 cattatggga cagtttgacc accccaatat cattcgactg gaaggagttg ttaccaaaag
      421 taagccagtt atgattgtca cagaatacat ggagaatggt tccttggata gtttcctacg
      481 taaacacgat gcccagttta ctgtcattca gctagtgggg atgcttcgag ggatagcatc
      541 tggcatgaag tacctgtcag acatgggcta tgttcaccga gacctcgctg ctcggaacat
      601 cttgatcaac agtaacttgg tgtgtaaggt ttctgatttc ggactttcgc gtgtcctgga
      661 ggatgaccca gaagctgctt atacaacaag aggagggaag atcccaatca ggtggacatc
      721 accagaagct atagcctacc gcaagttcac gtcagccagc gatgtatgga gttatgggat
      781 tgttctctgg gaggtgatgt cttatggaga gagaccatac tgggagatgt ccaatcagga
      841 tgtaattaaa gctgtagatg agggctatcg actgccaccc cccatggact gcccagctgc
      901 cttgtatcag ctgatgctgg actgctggca gaaagacagg aacaacagac ccaagtttga
      961 gcagattgtt agtattctgg acaagcttat ccggaatccc ggcagcctga agatcatcac
     1021 cagtgcagcc gcaaggccat caaacctt
//
