LOCUS       sequence-159268-         986 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of MARK3. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 986)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: MARK3_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..986
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcag atgaacaacc tcacatcgga
      181 aactacagac tgttgaaaac aatcggcaag gggaattttg caaaagtaaa attggcaaga
      241 catatcctta caggcagaga ggttgcaata aaaataattg acaaaactca gttgaatcca
      301 acaagtctac aaaagctctt cagagaagta agaataatga agattttaaa tcatcccaat
      361 atagtgaagt tattcgaagt cattgaaact gaaaaaacac tctacctaat catggaatat
      421 gcaagtggag gtgaagtatt tgactatttg gttgcacatg gcaggatgaa ggaaaaagaa
      481 gcaagatcta aatttagaca gattgtgtct gcagttcaat actgccatca gaaacggatc
      541 gtacatcgag acctcaaggc tgaaaatcta ttgttagatg ccgatatgaa cattaaaata
      601 gcagatttcg gttttagcaa tgaatttact gttggcggta aactcgacac gttttgtggc
      661 agtcctccat acgcagcacc tgagctcttc cagggcaaga aatatgacgg gccagaagtg
      721 gatgtgtgga gtctgggggt cattttatac acactagtca gtggctcact tccctttgat
      781 gggcaaaacc taaaggaact gagagagaga gtattaagag ggaaatacag aattcccttc
      841 tacatgtcta cagactgtga aaaccttctc aaacgtttcc tggtgctaaa tccaattaaa
      901 cgcggcactc tagagcaaat catgaaggac aggtggatca atgcagggca tgaagaagat
      961 gaactcaaac catttgttga accaga
//
