LOCUS       sequence-159311-         912 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon
            (for native or N-terminal fusions), G12V mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 912)
  AUTHORS   Esposito
  TITLE     RAS mutant collection V2.0
  JOURNAL   unpublished
REFERENCE   2  (bases 1 to 912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "unpublished"
COMMENT     Sequence Label: Hs.HRAS G12V
FEATURES             Location/Qualifiers
     source          1..912
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    25..124
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             join(124..156,157..159,160..693)
                     /codon_start=1
                     /product="human oncoprotein generated by the G12V mutation 
                     in the small GTPase H-Ras"
                     /label=H-Ras (G12V)
                     /translation="MTEYKLVVVGAVGVGKSALTIQLIQNHFVDEYDPTIEDSYRKQVV
                     IDGETCLLDILDTAGQEEYSAMRDQYMRTGEGFLCVFAINNTKSFEDIHQYREQIKRVK
                     DSDDVPMVLVGNKCDLAARTVESRQAQDLARSYGIPYIETSAKTRQGVEDAFYTLVREI
                     RQHKLRKLNPPDESGPGCMSCKCVLS"
     protein_bind    complement(692..791)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 aattcgagct cggtccgagg atccgaaata atgattttat tttgactgat agtgacctgt
       61 tcgttgcaac aaattgataa gcaatgcttt tttataatgc caactttgta caaaaaagtt
      121 ggcatgacgg aatataagct ggtggtggtg ggcgccgtcg gtgtgggcaa gagtgcgctg
      181 accatccagc tgatccagaa ccattttgtg gacgaatacg accccactat agaggattcc
      241 taccggaagc aggtggtcat tgatggggag acgtgcctgt tggacatcct ggataccgcc
      301 ggccaggagg agtacagcgc catgcgggac cagtacatgc gcaccgggga gggcttcctg
      361 tgtgtgtttg ccatcaacaa caccaagtct tttgaggaca tccaccagta cagggagcag
      421 atcaaacggg tgaaggactc ggatgacgtg cccatggtgc tggtggggaa caagtgtgac
      481 ctggctgcac gcactgtgga atctcggcag gctcaggacc tcgcccgaag ctacggcatc
      541 ccctacatcg agacctcggc caagacccgg cagggagtgg aggatgcctt ctacacgttg
      601 gtgcgtgaga tccggcagca caagctgcgg aagctgaacc ctcctgatga gagtggcccc
      661 ggctgcatga gctgcaagtg tgtgctctcc taatcaactt tcttgtacaa agttggcatt
      721 ataggaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag tcaaaataaa
      781 atcattattt gcatgcaagc ttagatcctc tagaccagcc aggacagaaa tgcctcgact
      841 tcgctgctac ccaaggttgc cgggtgacgc acaccgtgga aacggatgaa ggcacgaacc
      901 cagtggacat aa
//
