LOCUS       sequence-159322-         781 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-222 of CHMP4A, Mutation modestly disrupts binding to 
            ALIX; Bacterial Expression Vector; TEV-cleavable GST-tag; Sundquist 
            Lab Internal ID: WISP06-206.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 781)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (205-222) E209A
            (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..781
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..35
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(167..189)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(334..352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        420..524
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(743..762)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 cgcgtggatc tccggaaaac ctgtatttcc agggccatat gcccaaagtg gatgcagatg
       61 aagaagcact aaagcagttg gctgagtggg tatcctgagg atcctcgaga attcatcgtg
      121 actgactgac gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
      181 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
      241 agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg
      301 atagcggagt gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg
      361 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
      421 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
      481 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      541 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      601 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      661 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      721 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc
      781 a
//
