LOCUS       sequence-159346-        1007 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of CDK2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1007)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: CDK2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1007
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             63..85
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             105..134
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             135..155
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ccggctccgg agagctcttt aattaagcgg ccgccctgca ggactcgagt tctagaaata
       61 attttgttta actttaagaa ggagatatac atatgaaatc ttctcaccat caccatcacc
      121 atcaccatca ccatgaaaac ctgtacttcc aatccaatgc aatggagaac ttccaaaagg
      181 tggaaaagat cggagagggc acgtacggag ttgtgtacaa agccagaaac aagttgacgg
      241 gagaggtggt ggcgcttaag aaaatccgcc tggacactga gactgagggt gtgcccagta
      301 ctgccatccg agagatctct ctgcttaagg agcttaacca tcctaatatt gtcaagctgc
      361 tggatgtcat tcacacagaa aataaactct acctggtttt tgaatttctg caccaagatc
      421 tcaagaaatt catggatgcc tctgctctca ctggcattcc tcttcccctc atcaagagct
      481 atctgttcca gctgctccag ggcctagctt tctgccattc tcatcgggtc ctccaccgag
      541 accttaaacc tcagaatctg cttattaaca cagagggggc catcaagcta gcagactttg
      601 gactagccag agcttttgga gtccctgttc gtacttacac ccatgaggtg gtgaccctgt
      661 ggtaccgagc tcctgaaatc ctcctgggct gcaaatatta ttccacagct gtggacatct
      721 ggagcctggg ctgcatcttt gctgagatgg tgactcgccg ggccctattc cctggagatt
      781 ctgagattga ccagctcttc cggatctttc ggactctggg gaccccagat gaggtggtgt
      841 ggccaggagt tacttctatg cctgattaca agccaagttt ccccaagtgg gcccggcaag
      901 attttagtaa agttgtacct cccctggatg aagatggacg gagcttgtta tcgcaaatgc
      961 tgcactacga ccctaacaag cggatttcgg ccaaggcagc cctggct
//
