LOCUS       sequence-159364-        1015 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This plasmid encodes the kinase domain of KC1G2. Intended for 
            co-expression with Lambda Phosphatase that accompanies this set to 
            enhance bacterial kinase expression. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1015)
  AUTHORS   Albanese SK, Parton DL, Isik M, Rodriguez-Laureano L, Hanson SM, 
            Behr JM, Gradia S, Jeans C, Levinson NM, Seeliger MA, Chodera JD
  TITLE     An open library of human kinase domain constructs for automated 
            bacterial expression.
  JOURNAL   Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081.
  PUBMED    30004690
REFERENCE   2  (bases 1 to 1015)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry. 2018 Jul 13. doi: 10.1021/acs.biochem.7b01081."
COMMENT     Sequence Label: KC1G2_HUMAN_D0
FEATURES             Location/Qualifiers
     source          1..1015
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             61..83
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             103..132
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             133..153
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 ggctccggag agctctttaa ttaagcggcc gccctgcagg actcgagttc tagaaataat
       61 tttgtttaac tttaagaagg agatatacat atgaaatctt ctcaccatca ccatcaccat
      121 caccatcacc atgaaaacct gtacttccaa tccaatgcaa acttccgcgt cggcaagaag
      181 atcggctgcg gcaacttcgg ggagctccgc ctaggaaaga atctctatac aaatgaatac
      241 gtggctatca aattggagcc gatcaagtcc cgggccccgc agctgcacct ggagtaccgg
      301 ttctacaagc agctcagcgc cacagagggc gtccctcagg tctactactt cggtccgtgc
      361 gggaattaca acgccatggt gctggagctg ctggggccca gcctggagga cctgttcgac
      421 ctgtgcgacc ggaccttcac gctcaagacg gtgctgatga tcgccatcca gctgatcacg
      481 cgcatggagt atgtgcacac caagagccta atctaccggg acgtgaagcc cgagaacttc
      541 ctggtgggcc gcccggggac caagcggcag catgccatcc acatcatcga cttcgggctg
      601 gccaaggagt acatcgaccc cgagaccaag aagcacatcc cgtaccgcga gcacaagagc
      661 ctgacgggca cggcgcgcta catgagcatc aacacgcacc tgggcaagga gcagagccgc
      721 cgcgacgacc tggaggcgct gggccacatg ttcatgtact tcctgcgcgg cagcctcccc
      781 tggcaggggc tcaaggccga cacgctcaag gagcggtacc agaagatcgg ggacaccaaa
      841 cgcgccacgc ccatcgaggt gctctgcgag aacttcccag aggagatggc cacgtacctg
      901 cgctatgtgc ggcgcctgga cttcttcgag aagcccgact atgactacct gcggaagctc
      961 ttcaccgacc tcttcgaccg cagtggcttc gtgttcgact atgagtacga ctggg
//
