LOCUS       sequence-159366-         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Residues 205-222 of CHMP4A, Bacterial Expression Vector; 
            TEV-cleavable GST tag; Sundquist Lab Internal ID: WISP06-60.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   McCullough J, Fisher RD, Whitby FG, Sundquist WI, Hill CP
  TITLE     ALIX-CHMP4 interactions in the human ESCRT pathway.
  JOURNAL   Proc Natl Acad Sci U S A. 2008 Jun 3;105(22):7687-91. doi: 
            10.1073/pnas.0801567105. Epub 2008 May 29.
  PUBMED    18511562
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.0801567105"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2008-06-3- 3"; volume: "105"; issue: "22"; pages: "7687-91"
COMMENT     Sequence Label: GST-CHMP4A (205-222) (pGEX2T(TEV))
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(108..130)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(275..293)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        361..465
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagaagcac taaagcagtt ggctgagtgg gtatcctgag gatcctcgag aattcatcgt
       61 gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg
      121 cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt
      181 cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc
      241 gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag
      301 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
      361 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
      421 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
      481 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
      541 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
      601 gaactggatc tcaacagcgg ta
//
