LOCUS       sequence-159385-         967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression vector encoding murine Pitx2 downstream of a 
            doxycycline inducible promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 967)
  AUTHORS   Vereide DT, Vickerman V, Swanson SA, Chu LF, McIntosh BE, Thomson JA
  TITLE     An expandable, inducible hemangioblast state regulated by fibroblast
            growth factor.
  JOURNAL   Stem Cell Reports. 2014 Dec 9;3(6):1043-57. doi: 
            10.1016/j.stemcr.2014.10.003. Epub 2014 Nov 13.
  PUBMED    25458896
REFERENCE   2  (bases 1 to 967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.stemcr.2014.10.003"; journalName: "Stem Cell Reports"; 
            date: "2014-12-9- 9"; volume: "3"; issue: "6"; pages: "1043-57"
COMMENT     Sequence Label: PB-Pitx2
FEATURES             Location/Qualifiers
     source          1..967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    69..93
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ccatccacgc tgttttgacc tccatagaag acaccgggac cgatccagcc tccgcggtgg
       61 cggccatcac aagtttgtac aaaaaagcag gcttgaagga attcggtacc atgaactgca
      121 tgaaaggccc gctgcccttg gagcaccgag cagccgggac taagctgtcg gccgcctcct
      181 cacccttctg tcaccatccc caggcgttag ccatggcttc ggtcctagct cctggccagc
      241 cccgctcctt ggactcctcc aaacatagac tggaggtgca tacaatctcc gatacttcca
      301 gccctgaagt cgcagagaaa gataagggcc agcaaggaaa gaatgaggat gtgggcgccg
      361 aggacccgtc caagaagaag cggcaacgcc ggcagaggac tcatttcact agccagcagc
      421 tgcaggagct ggaagccact ttccagagaa accgctaccc agacatgtcc actcgcgaag
      481 aaatcgccgt gtggaccaac cttacggaag cccgagtccg ggtttggttc aagaatcgcc
      541 gggccaaatg gagaaagcgg gaacgcaacc agcaggccga gctgtgcaag aatggctttg
      601 ggccgcagtt caacgggctc atgcagccct acgatgacat gtaccccggc tattcgtaca
      661 acaattgggc tgccaagggc ctcacgtcag cgtctctgtc cacnnagagc ttccccttct
      721 tcaactccat gaacgtcaat cccctgtcct ctcagagtat gttttccccg cccaactcca
      781 tctcatctat gagtatgtcg tccagcatgg tgccctccgc ggtgaccggc gtcccgggct
      841 ccagcctcaa tagcctgaat aacttgaaca acctgagcag cccgtcgctg aattccgcgg
      901 tgcccacgcc cgcctgtcct tacgcgccgc cgactcctcc gtacgtttat agggacacat
      961 gtaactc
//
