LOCUS       sequence-159406-         950 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Translation reporter: Encodes KDM5B (1544 aa) fused to smFLAG 
            (Spaghetti Monster FLAG: 10 copies of an epitope tag FLAG). .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 950)
  AUTHORS   Morisaki T, Lyon K, DeLuca KF, DeLuca JG, English BP, Zhang Z, Lavis
            LD, Grimm JB, Viswanathan S, Looger LL, Lionnet T, Stasevich TJ
  TITLE     Real-time quantification of single RNA translation dynamics in 
            living cells.
  JOURNAL   Science. 2016 Jun 17;352(6292):1425-9. doi: 10.1126/science.aaf0899.
            Epub 2016 May 5.
  PUBMED    27313040
REFERENCE   2  (bases 1 to 950)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1126/science.aaf0899"; journalName: "Science"; date: 
            "2016-06-17- 17"; volume: "352"; issue: "6292"; pages: "1425-9"
COMMENT     Sequence Label: pUB_smFLAG_KDM5B_MS2
FEATURES             Location/Qualifiers
     source          1..950
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             152..163
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 catctgtaag gtgcggccgc cgccggattg gcagccacca tttgcatgtg atgttgataa
       61 acttcatttt acgccacgta tccagagact gaatgaattg gaggcccaaa ctcgtgtaaa
      121 attgaatttc ttggaccaga ttgcaaagta ctgggagtta cagggaagta ctctgaaaat
      181 tccacatgtg gagaggaaga tcttggactt atttcagctt aataagttag ttgcagaaga
      241 aggtggattt gcagttgttt gcaaggatag aaaatggacc aaaattgcta ccaagatggg
      301 gtttgctcct ggcaaagcag tgggctcaca tatcagaggg cattatgaac gaattctcaa
      361 cccctacaac ttattcctgt ccggagacag cctaaggtgt ttgcagaagc caaacctgac
      421 cacagacact aaggacaagg agtacaaacc ccatgatatt ccccagaggc agtctgtgca
      481 gccttcggaa acgtgccccc cagcccgacg agcaaaacgc atgagagcag aggccatgaa
      541 tattaaaata gaacccgagg agacaacaga agccagaact cataatctga gacgtcgaat
      601 gggttgtcca actccaaaat gtgaaaatga gaaagaaatg aagagtagca tcaagcaaga
      661 acctattgag aggaaagatt atattgtaga aaatgagaag gaaaagccca agagtcgatc
      721 taaaaaagcc accaatgctg tggacctgta tgtctgtctt ttatgtggca gtggcaatga
      781 tgaagaccgg ctactgttgt gtgatggctg tgatgacagt taccatacct tttgcttgat
      841 cccacctctc catgatgttc ccaagggaga ctggaggtgt cctaagtgtt tggctcagga
      901 atgtagtaag ccacaagaag catttggctt tgaacaagca gccagggact
//
