LOCUS       sequence-159537-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Pmex-5:GFP::H2B::PEST::cul-4 3'UTR reporter construct used for 
            microinjection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Kaymak E, Farley BM, Hay SA, Li C, Ho S, Hartman DJ, Ryder SP
  TITLE     Efficient generation of transgenic reporter strains and analysis of 
            expression patterns in Caenorhabditis elegans using Library MosSCI.
  JOURNAL   Dev Dyn. 2016 Jun 13. doi: 10.1002/dvdy.24426.
  PUBMED    27294288
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2016 Jun 13. doi: 10.1002/dvdy.24426."
COMMENT     Sequence Label: pCFJ150-cul4
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(138..162)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(652..672)
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 tgacacattg atcctagcag aagcacaggc tgcagggtga cggtccatcc cgctctcctg
       61 ggcacaagac atgggcagcg tgccatcatc ctgcgccgcc accgccggcg ggaagccatg
      121 gctaagtcta gacatggagc ctgctttttt gtacaaactt gtcattctct gtctgaaaca
      181 ttcaattgat tatcgataaa attaattaag agagaataca atgagaaaac ggaaaatcga
      241 gcaggatttt ggggaaagtc ggggaatttt tgctatttaa gctttcttta acaaagaaac
      301 tgcatttttc catgagatta ccatgaaatt tgcgttaaat gggtgaattt ataacattta
      361 cggctagaaa ttaattttaa aaaagagaat aaacgaaaaa ttattcgaga aaacgtcttt
      421 gaagcagaaa tgaaatgaaa aaacaaccgc cagcgctgac aaaaatgatc aaacaaacgg
      481 agtacggtat acgcggtaag acccaaatga attttaattc ggtgcgcctt taaaaaatgt
      541 atttttttaa tttctttatc cgtgggccca aatgtatttt tttcttgtgg tttgatgtaa
      601 aaattgggtt atattatttg ttttatggtt taatttttta aaaactgata tcaacttttc
      661 tatacaaagt tgatagcttg gcgtaatcat ggtcatagct gttcctgcct aggtgagctc
      721 tggtaccctc tagtcaaggc cttaagtgag tcgtattacg tagcccgggc ctagttctag
      781 acattctcta atgaaaaaat ctttcagttg aaattga
//
