LOCUS       sequence-159547-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  sgRNA targeting YFP with additional bases 5' end like pCas34, but 
            from pJ23119 in the NS1 locus with kanamycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Gordon GC, Korosh TC, Cameron JC, Markley AL, Begemann MB, Pfleger 
            BF
  TITLE     CRISPR interference as a titratable, trans-acting regulatory tool 
            for metabolic engineering in the cyanobacterium Synechococcus sp. 
            strain PCC 7002.
  JOURNAL   Metab Eng. 2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007.
  PUBMED    27481676
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Metab Eng. 
            2016 Jul 29. pii: S1096-7176(16)30062-3. doi: 
            10.1016/j.ymben.2016.07.007."
COMMENT     Sequence Label: pCas45
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(56..90)
                     /label=J23119 promoter
                     /note="bacterial promoter (Registry of Standard Biological 
                     Parts BBa_J23119)"
ORIGIN
        1 ttaacttgct atttctagct ctaaaacgcg agggcgaggg cgatgccagt taacagctag
       61 cattatacct aggactgagc tagctgtcaa gaattccgtt attgatacaa aattaactca
      121 agaaaaatag tgtgaactaa aagatacagt aggatatttt aatacagcaa tttcatgcct
      181 atttgacgaa tttctaagta tagtaataaa aacacttgga gtcagtaaaa aaacgactgt
      241 gttttagtgc tttagaatgc aaagacaggc gtcactgtaa gctgaagatt ttttagccgc
      301 tcttttttta ttttcatata gcctttgaga tatttttcct cactattcaa aatattatat
      361 ttactcagtt tttaagtaat tagcagacaa ttctacatca tcatctagct tattgtgagt
      421 cgattcattt tgtcgggctt catttgtgct agaagcccgt ccttgtgaaa ccaaccgaac
      481 tgcagaacat tgccgcgcca gtactggata aacctctgga tctacttgaa tgcctgaaga
      541 aatcatgtca gcacaaagtt tgactgtttc cgctgaagat tggatttgaa actttgctaa
      601 ttcggcttgt tctctttcca gtagggtagt gctgaaatct ctacagttgc tgcccacttg
      661 accaccgagg ggcacaataa tggcaccgga aagagcgtag gaatttgaac tctccccatc
      721 aaatgtattg ccatttgcag agccactgag cacaaaggaa gggcggggac aaacgatccc
      781 tctaaaaccg taggtggcac tttgacctaa gttgtacaca gcggtttggt tgactacccc
      841 agtcgcacta ttccccaatg aattttcatt attcatcgta ttactttgat cttgcatcgt
      901 ttgagccgct ccgtaaatag gagtcaggaa
//
