LOCUS       sequence-159576-         889 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Inducible expression of redox sensitive GFP in bacteria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 889)
  AUTHORS   Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, 
            Remington SJ
  TITLE     Investigating mitochondrial redox potential with redox-sensitive 
            green fluorescent protein indicators.
  JOURNAL   J Biol Chem. 2004 Mar 26;279(13):13044-53. Epub 2004 Jan 13.
  PUBMED    14722062
REFERENCE   2  (bases 1 to 889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-03-26"; volume: "279(13)"; pages: "13044-53. 
            Epub 2004 Jan 13"
COMMENT     Sequence Label: pRSETB-ro1GFP
FEATURES             Location/Qualifiers
     source          1..889
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..784)
                     /codon_start=1
                     /product="S65T variant of Aequorea victoria green 
                     fluorescent protein (Heim et al., 1995)"
                     /label=GFP (S65T)
                     /note="excitable with blue light"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FISTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(111..131)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     726..754
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     CDS             complement(join(803..817,818..826))
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     CDS             complement(830..862)
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             complement(866..883)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ttgttagcag ccggatcaag cttcgaattc catggtacca gctgcagatc tcgagctcgg
       61 atccttatta tttgtatagt tcatccatgc catgtgtaat cccagcagct gttacaaact
      121 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca gagcatgtgg
      181 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattggagta ttttgttgat
      241 aatggtctgc tagttgaacg cttccatctt caatgttgtg tctaattttg aagttaactt
      301 tgattccatt cttttgtttg tctgccatga tgtatacatt gtggcagtta tagttgtatt
      361 ccaatttgtg tccaagaatg tttccatctt ctttaaaatc aatacctttt aactcgattc
      421 tattaacaag ggtatcacct tcaaacttga cttcagcacg tgtcttgtag ttcccgtcat
      481 ctttgaaaaa tatagttctt tcctgtacat aaccttcggg catggcactc ttgaaaaagt
      541 catgccgttt catatgatct gggtatcttg aaaagcattg aacaccataa ctgaaagtag
      601 tgacaagtgt tggccatgga acaggtagtt ttccagtagt ggaaataaat ttaagggtaa
      661 gttttccgta tgttgcatca ccttcaccct ctccactgac agaaaatttg tgcccattaa
      721 catcaccatc taattcaaca agaattggga caactccagt gaaaagttct tctcctttac
      781 tcatgaattc agcgggggga tccttatcgt catcgtcgta cagatcccga cccatttgct
      841 gtccaccagt catgctagcc ataccatgat gatgatgatg atgagaacc
//
