LOCUS       sequence-159593-         971 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  storage vector for BASIC part: GFP expression cassette.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 971)
  AUTHORS   Storch M, Casini A, Mackrow B, Fleming T, Trewhitt H, Ellis T, 
            Baldwin GS
  TITLE     BASIC: A New Biopart Assembly Standard for Idempotent Cloning 
            Provides Accurate, Single-Tier DNA Assembly for Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2015 Apr 16.
  PUBMED    25746445
REFERENCE   2  (bases 1 to 971)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Apr 16."
COMMENT     Sequence Label: BASIC_10_J23101-RBS32-GFP-B0015
FEATURES             Location/Qualifiers
     source          1..971
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        41..75
                     /label=BBa_J23101
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.7"
     CDS             103..816
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     terminator      845..916
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      932..959
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 ggctcgagtt tttcagcaag attctggtgg gtctctgtcc tttacagcta gctcagtcct
       61 aggtattatg ctagctacta gagtcacaca ggaaagtact agatgcgtaa aggcgaagaa
      121 ctgttcacgg gcgtagttcc gattctggtc gagctggacg gcgatgtgaa cggtcataag
      181 tttagcgttc gcggtgaagg tgagggcgac gcgaccaacg gcaaactgac cctgaagttc
      241 atctgcacca ccggtaaact gccggtgcct tggccgacct tggtgacgac gttgacgtat
      301 ggcgtgcagt gttttgcgcg ttatccggac cacatgaaac aacacgattt cttcaaatct
      361 gcgatgccgg agggttacgt ccaggagcgt accatttcct tcaaggatga tggcacttac
      421 aaaactcgcg cagaggttaa gtttgaaggt gacacgctgg tcaatcgtat cgaattgaag
      481 ggtatcgact ttaaagagga tggtaacatt ctgggccata aactggagta taacttcaac
      541 agccataatg tttacattac ggcagacaag caaaagaacg gcatcaaggc caatttcaag
      601 attcgccaca atgttgagga cggtagcgtc caactggccg accattacca gcagaacacc
      661 ccaattggtg acggtccggt tttgctgccg gataatcact atctgagcac ccaaagcgtg
      721 ctgagcaaag atccgaacga aaaacgtgat cacatggtcc tgctggaatt tgtgaccgct
      781 gcgggcatca cccacggtat ggacgagctg tataagcgtc cgtaataata ctagagccag
      841 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
      901 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
      961 ttataggctc g
//
