LOCUS       sequence-160058-         956 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of Zdk1 anchored to mitochondria.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 956)
  AUTHORS   Wang H, Vilela M, Winkler A, Tarnawski M, Schlichting I, Yumerefendi
            H, Kuhlman B, Liu R, Danuser G, Hahn KM
  TITLE     LOVTRAP: an optogenetic system for photoinduced protein 
            dissociation.
  JOURNAL   Nat Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926.
  PUBMED    27427858
REFERENCE   2  (bases 1 to 956)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2016 Jul 18. doi: 10.1038/nmeth.3926."
COMMENT     Sequence Label: pTriEx-NTOM20-mVenus-Zdk3
FEATURES             Location/Qualifiers
     source          1..956
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     88..107
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(197..220)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(227..259)
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             complement(299..322)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(329..349)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(356..373)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(587..838)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(629..650)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
       61 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatcg atctcagtgg
      121 tatttgtgag ccagggcatt ggccacacca gccaccacct tctgataggc agcctgcacc
      181 tgaggttaat cacttagtga tggtgatggt gatggtggtg ctcgagatcc tcggggtctt
      241 ccggggcgag ttctggctgg cttgcacgtg tatacagctg tgcggccgca agcttttatt
      301 tgtcgtcgtc gtccttgtag tcgctgccgc cctggaaata cagattttca ccgccatgat
      361 gatgatgatg atggctacca ccagaaccac cttttggggg ctgggcaccg ttcagttttt
      421 tggcttcggc cagcagattg gcgctctgag atggatcatt agtcaggcta ctgatgaagg
      481 caaacttctg ctcccaattc aggtttggca gccaatagat ttcctgccgg gccaccagca
      541 cttctttatt gaatttgtta tccaccatgg atccacttcc agaacccttg tacagctcgt
      601 ccatgccgag agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct
      661 tctcgttggg gtctttgctc agtttggact ggtagctcag gtagtggttg tcgggcagca
      721 gcacggggcc gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgccgc
      781 cgtcctcgat gttgtggcgg atcttgaagt ttgccttgat gccgttcttc tgcttgtcgg
      841 cggtgatata gacgttgtgg ctgttgtagt tgtactccag cttgtgcccc aggatgttgc
      901 cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccc
//
