LOCUS       sequence-160134-         947 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses 6xHis tagged PP3359 under pBAD regulation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 947)
  AUTHORS   Lo TM, Chng SH, Teo WS, Cho HS, Chang MW
  TITLE     A Two-Layer Gene Circuit for Decoupling Cell Growth from Metabolite 
            Production.
  JOURNAL   Cell Syst. 2016 Aug;3(2):133-43. doi: 10.1016/j.cels.2016.07.012.
  PUBMED    27559924
REFERENCE   2  (bases 1 to 947)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cels.2016.07"; journalName: "Cell Syst"; date: "2016-08";
            volume: "3"; issue: "2"; pages: "133-43"
COMMENT     Sequence Label: pBbE8k-PP3359-6xHis
FEATURES             Location/Qualifiers
     source          1..947
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             65..76
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             551..568
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      586..657
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      673..700
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      739..810
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      826..853
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 acgcttttta tcgcaactct ctactgtttc tccatacccg tttttttggg aattcaagag
       61 atctaaagag gagaaaggat ctatggctag gtctgcccgt agtaccgacg acgcttgcgt
      121 ggctgctcct gtgggagaag gggtgcttga agacttgatc ggctacgcct tgcgacgcgc
      181 gcaattgaag ctgtttcaga accttattgc ccggctctcg gcccatgacc tgcgcccggc
      241 ccaattttcc gccctggcga tcatcgacca gaaccccggg ctgatgcagg ccgacctggc
      301 gcgtgcgttg gcaatcgacc ccccgcaagt cgtgccaatg ctgaacaaac tggaagagcg
      361 cgcgctggcc gtgcgcgtgc ggtgcaaacc ggacaagcgc tcgtatggga ttttcctgag
      421 caaatcgggc gaggccctgt tgaaggagtt gaagcacatc gccgccgaca gcgatcacca
      481 ggcgacatcc aacctctcgg atgacgaaag gactgaactg ttgaggttat tgaagaaaat
      541 ctaccgggac catcatcatc atcatcatta aggatctcca ggcatcaaat aaaacgaaag
      601 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
      661 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttataggat ccaaactcga
      721 gtaaggatct ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
      781 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
      841 gggcctttct gcgtttatac ctagggcgtt cggctgcggc gagcggtatc agctcactca
      901 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaa
//
