LOCUS       sequence-160155-         872 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Constitutively active NFAT1, mutated to interfere selectively with 
            the NFAT:AP-1 interactio. Contains Puromycin selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 872)
  AUTHORS   Martinez GJ, Pereira RM, Aijo T, Kim EY, Marangoni F, Pipkin ME, 
            Togher S, Heissmeyer V, Zhang YC, Crotty S, Lamperti ED, Ansel KM, 
            Mempel TR, Lahdesmaki H, Hogan PG, Rao A
  TITLE     The Transcription Factor NFAT Promotes Exhaustion of Activated CD8 T
            Cells.
  JOURNAL   Immunity. 2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006.
  PUBMED    25680272
REFERENCE   2  (bases 1 to 872)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Immunity. 
            2015 Feb 6. pii: S1074-7613(15)00032-1. doi: 
            10.1016/j.immuni.2015.01.006."
COMMENT     Sequence Label: CA-RIT-NFAT1 (PGK-Puro)
FEATURES             Location/Qualifiers
     source          1..872
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(28..47)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     421..444
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     protein_bind    496..520
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(594..614)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 agaggagaac agcgcggcag acgtgcgctt ttgaagcgtg cagaatgccg ggcctccgga
       61 ggaccttcgg gcgcccgccc cgcccctgag cccgcccctg agcccgcccc cggacccacc
      121 ccttcccagc ctctgagccc agaaagcgaa ggagcaaagc tgctattggc cgctgcccca
      181 aaggcctacc cgcttccatt gctcagcggt gctgtccatc tgcacgagac tagtgagacg
      241 tgctacttcc atttgtcacg tcctgcacga cgcgagctgc ggggcggggg ggaacttcct
      301 gactagggga ggagtagaag gtggcgcgaa ggggccacca aagaacggag ccggttggcg
      361 cctaccggtg gatgtggaat gtgtgcgagg ccagaggcca cttgtgtagc gccaagtgcc
      421 cagcggggct gctaaagcgc atgctccaga ctgccttggg aaaagcgcct cccctacccg
      481 gtagaattcg ttatcaccac tttgtacaag aaagctgggt ctagataaag caagtaaaac
      541 ctctacaaat gtggtatggc tgattatgat ctagagtcgc ggcccgggtc ttccactttg
      601 cgtttttttt tcggcggggt gtacgtctga tttcgcgagg gaggtcctga aaactccttc
      661 ctgatgattt cattaacgtc atccaggtag gtctggtcca ggttctgtcc ctgtttgacg
      721 gtcactcccg tgggcagagc ttccttctgg tcactgggtc cgtttttggc agctctttgg
      781 ctcggggcag tctgttgttg gatgactgtg gggtaggcgc ccgggctcag cctctgacct
      841 tggttgatgg gaggcgggcc aggcctggca ga
//
