LOCUS       sequence-160164-        5578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries NEW operon 6 final.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5578)
  AUTHORS   Javidpour P, Deutsch S, Mutalik VK, Hillson NJ, Petzold CJ, Keasling
            JD, Beller HR
  TITLE     Investigation of Proposed Ladderane Biosynthetic Genes from Anammox 
            Bacteria by Heterologous Expression in E. coli.
  JOURNAL   PLoS One. 2016 Mar 14;11(3):e0151087. doi: 
            10.1371/journal.pone.0151087. eCollection 2016.
  PUBMED    26975050
REFERENCE   2  (bases 1 to 5578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-14"; volume: "11(3):e0151087. doi"; pages: " 
            10.1371/journal.pone.0151087. eCollection 2016"
COMMENT     Sequence Label: pPJ170(JPUB_006771)
FEATURES             Location/Qualifiers
     source          1..5578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(150..695)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(230..249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(809..903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(934..1728)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1045..1064)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1655..1674
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
       61 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
      121 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
      181 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
      241 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
      301 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
      361 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
      481 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
      541 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
      601 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
      661 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
      721 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
      781 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
      841 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      901 gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      961 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     1021 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     1081 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     1141 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     1201 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     1261 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     1321 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     1381 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     1441 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     1501 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     1561 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1621 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1681 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     1741 tcatcctgtc tcttgatcag atcatgatcc cctgcgccat cagatccttg gcggcaagaa
     1801 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     1861 ttccgaattc atgagatctc gtgctttcta tgagtcgttg ctgcataact tgacatccct
     1921 atcagtgata gacataatta tttcatgata tcaaactcga gtttaagaag gagatataca
     1981 tatgagtggc cgcatcctga tcaccggtat tggttttatc accccgatta aaccatttca
     2041 aggtgtgaat gagttctggc atgcgctgtg tagtggccaa gacctgatta agaaaacccg
     2101 tctgccactg ctggaaattg acggtgaatg gccgatggcg gaaatcagcc tgagttgtat
     2161 tacgcaaacc gtgcaaccgg aagacaaatt tcaatacctg atgaaacgtg cgctgaatat
     2221 ggcgatcgag gacgccaatc tgaaagattg tagtaatatc ggtttcagta tgggtacggt
     2281 tctgggcaac atgctgctga aggagaagaa actggcggat gccaaaaagc agaaaaagga
     2341 atactgtggt gaacaagata gtctgagtaa tgtgacgctg caatttagta gcctgtacaa
     2401 cctgaacggt ccaaaataca ccgtgagtac cgcgtgtacg agcggtacgg atgcgattgg
     2461 tattgcggcg cgtagcattc tgagtggtaa aacggatatt atgattgccg gtggtgccga
     2521 tatcctgagt gatttcgcga ttattggctt tcatgccctg caagcgatta ccaccgataa
     2581 agtgcgtcca tttgataaga atcgtagtgg tctggcgctg ggtgagggtg cgagttttgt
     2641 ggtgctggag agtgaaaaaa gtgccgccaa gcgtaatgcg aaagtgtacg gtcgtgtgat
     2701 gggttacgcc agtcgtgccg acgcgaatca tctgaccggc ccacatcgtg aaggtcgtgg
     2761 cctggccgag gcgattgacc gtgccatggg tcaaagtggc gtgaaaccag gtgatattaa
     2821 ctacattaac gcgcatggta cgggtaccgt ttacaatgat ctgatggaga ccaaggccat
     2881 taaacgtgtg tttggtcgtc tggcctacga tattccaatt agtagtacca aaagtatgct
     2941 gggccacagt ctgggtgcgg cgggtgccat cgaagcgatc tgttgtctgc tggccatcaa
     3001 aaacggcatt gtgccaccaa ccattaactt tcaagaatgt gatccggact gtgacctgga
     3061 ttacacccca aatattgccc gtaatcatca tgtgaaaatg gccatgagcc tgagcgccgg
     3121 tttcggcggt caaaatagtg ccattatcct gggtggcgcg taaaggaggt tttctaatgg
     3181 atcgtattgt gctgaccggt gttggcccac tgaccccgat tggtaaaggt aaagaagatt
     3241 tttggaatgc cgtggttaat aactttccag gcatcaagaa aatcaccaaa ttcatgccag
     3301 atcaagagtt ctatggcggt gagatcaacg atattcattt cgatgaatac atcccagata
     3361 cgaagtttcg tcgtgcgacg gaaattagca aatttacgat gagtgcggcc aaaatggcga
     3421 tggatgatgc ccaagtggat agtccgaaca ttaagaatct gggcctggtg gtgggtctga
     3481 cgcatggcgc gctgaattac acccaaagtt accatgagct gctggtgacc gaaggcccgg
     3541 agagtgcgag cccgattctg tttagtgaca gtattctgaa cgccccagcc ggtaatacga
     3601 gtatttgtct gggtatccat ggtccagtgc ataccctgat cggcggtagt gtgaccacga
     3661 ttcgtgccat catgctggcc gttcaactgc tggcgaccaa aaagatggaa aagagcgtga
     3721 ttgccagtgc cgaagaactg aataagttta gtctgtattg ttactatcgt ctgggcatta
     3781 acacgctgag tgaaggtgcc ggtgcgctgc tgattgagcc agaagacacc aagaacaatc
     3841 aagtgccata ctgttacatt agtggttttg ccagccaaac caatccaagt aacctgcaaa
     3901 gcgccctgga agaaagcgtt gaaaccgccc tgaaaaaagc caatctgaag gccaaagatg
     3961 tggatctgct gatgaccagt ccaagtttcc caaaagttca acaactggac gcctacgaaa
     4021 ttccaatggg tagtctggat agcattacgg gtaacgcctt tgccgtgagt accatttgga
     4081 acgttatcct gagtgccctg tgtatccgtc atggtgccat tccactgccg atcctgaaga
     4141 atagtgcccc aatcaataat gaaattcgta atgtgatgat ttgtgccacc gagagcgagg
     4201 gtgtggcggg tgttctgatt ctgagcaaaa tcagttaaag gaggttttct aatgaaaaaa
     4261 cgtgttgtga ttaccggcat gggtatcatc acggcccacg gtatcggtaa ggagatcacc
     4321 tggaaaaaga ttgtgagtgg tgcgagcggc atcaaagaaa tcgagagttt tgatagtagt
     4381 aaatacccgg gcaagtgtgg tggcgaagcg aaacaattta cgagtgccag tgaaattcgc
     4441 aacctgaatg cggcgcgtct ggatcgcgcc agccatctgc tgatccatgc cagtcgtgaa
     4501 gcgctgctgg aaagtaacgt gctgccgtac attaagaaca cgaacatcct gctgagcctg
     4561 ggcacgaccc tgggtggtat gattagtggt gagctgtttc ataaagaagt gctgaagaaa
     4621 ggtctgaaaa aggcgcgtat cagtctgctg acggactacc tggccaattg tcaggccatt
     4681 aacctgttcc gcgagttcca actgaagggc aattacaata tctttagtaa tgcctgtgcc
     4741 agcggtacca acgccattgg ctgtgcctac aatagtattc aaagtggtct gtacaaaatt
     4801 gccatttgtg gcggttatga cacgatgagt gaatttacct ttgcgggctt cagtagtctg
     4861 atggcgatga ccccaaccaa atgtcgtcca tttgacaaaa atcgcaacgg tctggttctg
     4921 ggcgagggcg tgggtattct gattctggaa gaactggaac atgcggttaa acgtaacgtt
     4981 cgcattctgg gtgagattat tggttacggt gaaagtagtg atgcctacca tatgaccagt
     5041 ccagacccaa ccggtgagtt tgcctttcgt gccattatga acgcgctgaa tgatgcggat
     5101 aatccaaaaa ttgattacat caatgcccat ggtacgggca ccatctataa cgacgcccaa
     5161 gagagcaatg cgattctgaa agcctttggt gaggaaacgc aaaacattcc agtgagtagt
     5221 attaaaccaa tggttggcca tatcctgggc ggtgcgggtg ccgtggaggc catcgttagc
     5281 ctgctgagta ttgaacgtaa agccgttcca ccaaacatta actactgtac cccagatcca
     5341 gtgtgtaatc tgaacattag taaggaaacc gtgtactgtg acatcaagac ggttctgagc
     5401 aatagtttcg gttttggcgg tacgaacgcc accctgatcc tgcgtgaata cacgtggatt
     5461 ggttaaggga actgccagac atcaaataaa acaaaaggct cagtcggaag actgggcctt
     5521 ttgttttatc tgttgtttgt cggtgaacac tctcccggga tcctaaaagc ttcctagg
//
