LOCUS       sequence-160169-        4733 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Carries NEW operon 10 final.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4733)
  AUTHORS   Javidpour P, Deutsch S, Mutalik VK, Hillson NJ, Petzold CJ, Keasling
            JD, Beller HR
  TITLE     Investigation of Proposed Ladderane Biosynthetic Genes from Anammox 
            Bacteria by Heterologous Expression in E. coli.
  JOURNAL   PLoS One. 2016 Mar 14;11(3):e0151087. doi: 
            10.1371/journal.pone.0151087. eCollection 2016.
  PUBMED    26975050
REFERENCE   2  (bases 1 to 4733)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2016-03-14"; volume: "11(3):e0151087. doi"; pages: " 
            10.1371/journal.pone.0151087. eCollection 2016"
COMMENT     Sequence Label: pPJ173(JPUB_006777)
FEATURES             Location/Qualifiers
     source          1..4733
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(16..33)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(150..695)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(230..249)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(809..903)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(934..1728)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1045..1064)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     1655..1674
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
ORIGIN
        1 gatatattcc gcttcctcgc tcactgactc gctacgctcg gtcgttcgac tgcggcgagc
       61 ggaaatggct tacgaacggg gcggagattt cctggaagat gccaggaaga tacttaacag
      121 ggaagtgaga gggccgcggc aaagccgttt ttccataggc tccgcccccc tgacaagcat
      181 cacgaaatct gacgctcaaa tcagtggtgg cgaaacccga caggactata aagataccag
      241 gcgtttcccc ctggcggctc cctcgtgcgc tctcctgttc ctgcctttcg gtttaccggt
      301 gtcattccgc tgttatggcc gcgtttgtct cattccacgc ctgacactca gttccgggta
      361 ggcagttcgc tccaagctgg actgtatgca cgaacccccc gttcagtccg accgctgcgc
      421 cttatccggt aactatcgtc ttgagtccaa cccggaaaga catgcaaaag caccactggc
      481 agcagccact ggtaattgat ttagaggagt tagtcttgaa gtcatgcgcc ggttaaggct
      541 aaactgaaag gacaagtttt ggtgactgcg ctcctccaag ccagttacct cggttcaaag
      601 agttggtagc tcagagaacc ttcgaaaaac cgccctgcaa ggcggttttt tcgttttcag
      661 agcaagagat tacgcgcaga ccaaaacgat ctcaagaaga tcatcttatt aatcagataa
      721 aatatttcta gatttcagtg caatttatct cttcaaatgt agcacctgaa gtcagcccca
      781 tacgatataa gttgttacta gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
      841 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
      901 gtccaagcga gctctcgaac cccagagtcc cgctcagaag aactcgtcaa gaaggcgata
      961 gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa agcacgagga agcggtcagc
     1021 ccattcgccg ccaagctctt cagcaatatc acgggtagcc aacgctatgt cctgatagcg
     1081 gtccgccaca cccagccggc cacagtcgat gaatccagaa aagcggccat tttccaccat
     1141 gatattcggc aagcaggcat cgccatgggt cacgacgaga tcctcgccgt cgggcatgcg
     1201 cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc tgatgctctt cgtccagatc
     1261 atcctgatcg acaagaccgg cttccatccg agtacgtgct cgctcgatgc gatgtttcgc
     1321 ttggtggtcg aatgggcagg tagccggatc aagcgtatgc agccgccgca ttgcatcagc
     1381 catgatggat actttctcgg caggagcaag gtgagatgac aggagatcct gccccggcac
     1441 ttcgcccaat agcagccagt cccttcccgc ttcagtgaca acgtcgagca cagctgcgca
     1501 aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc tcgtcctgca gttcattcag
     1561 ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc ccctgcgctg acagccggaa
     1621 cacggcggca tcagagcagc cgattgtctg ttgtgcccag tcatagccga atagcctctc
     1681 cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt tcaatcatgc gaaacgatcc
     1741 tcatcctgtc tcttgatcag atcatgatcc cctgcgccat cagatccttg gcggcaagaa
     1801 agccatccag tttactttgc agggcttccc aaccttacca gagggcgccc cagctggcaa
     1861 ttccgaattc atgagatctc gtgctttcta tgagtcgttg ctgcataact tgacatccct
     1921 atcagtgata gacataatta tttcatgata tcaaactcga gtttaagaag gagatataca
     1981 tatgaaagat tttgatgtga ttgttatcgg tagtggtatt ggtggtctga ttagtgcggg
     2041 caccctggtg agcaaaggca tgaaaacgct gctggtggag aaaaacaaga tcccaggcgg
     2101 tctgctggcg agcttcaaac gtaaaggttt tctgtttaac agtgcggttg attgtattag
     2161 tggtgtggcc aaaggcggtt acattagtaa tgttctggag agcctggatg ttgacagtga
     2221 aattaacttc gttaagctga atccaattcg ttttagcatt tttccggata ttcgtattcc
     2281 ggtggacagt aatatgaacg tgtatattga acgcctgatt caactgtttc caagtgaagc
     2341 cagcggtatc aagaagctgt tcaactttat ggatggtagc tttacgctgg ttcaaagtat
     2401 gctggatagc tttctgagcg gtaaaccggg tctgggcaaa gcgacccatg agatttttga
     2461 gctgcgcaat atcagttaca aggatctgct gcgtaagtat atcaccaatt atcgcctgac
     2521 gatggttctg agtgatcgtt gtccattcat cggcctgcca ccaagtcgtg tgagtgcctt
     2581 tagtatggtt aacatgatta tgagctactt taaactgggt gcctaccgtc caatcggtgg
     2641 ctttcaaaaa ctggccgata ttctggtgaa gggtattgag aacaagggcg gcatggtgct
     2701 gctgggtaac ggtgcggata agattctgct ggaaaacaat aatcgctgtt atggtgtgcg
     2761 ttgcgaaaac ggcaatgaat acacgagtga gttcgttgtg agtagtgttg atagtcatca
     2821 tacgttcggc gacctgctgg gtcgtgaatt tgagagtatc ccgacggaaa tgcgtaaaaa
     2881 gacgggtatc agtaccagct tttttgtggt gtatgccggt gttaaaggtg aagtgaccga
     2941 acatagtagc gttggttact tcccgagtta cgatatggag agtttcttca gtccaaaaag
     3001 cagctttaaa aatgacagta cgattggtgt gaccgttgcc agcatggagg ataaactgct
     3061 ggccccagac gcctgcaaca cggttgttct gcacgagatg gccgaagcga gtgacaaaga
     3121 gatcgataaa agcgtgtgta cgggcattgt gattaagaaa gccgagaaga ttattccagg
     3181 tctgaaggac aaaatcattg tgctggacag cgcgaccccg aaaagtatcg agcgttacac
     3241 cggtaacttc aatggtagtg cgttcggttg gaaccaaatc ccgggcttca agaatgtgaa
     3301 aggttacggc atcaacaatc tgtacattgc gggtcactgg ggtgagattg gcggtggcgt
     3361 tctggcggcg gcgtatagcg gtgccaaagc cgcgaaagaa attctgacca aggagagtgt
     3421 gacgaatggc atttaaagga ggttttctaa tgaaaaaagc ctttccaagt ccaccagaaa
     3481 ttctgccgca tgcctaccca ttcattatga tcgataagat tatcaagttt gaggaaaaga
     3541 ttagtattgt gtgtatcaaa aacgttagcc tgaacgatga atacctgggt ggtcattacc
     3601 aaaaaaacac caattttcca gccctgccac aagtgctggt gctggaagcc atggtgcaaa
     3661 gtagtggtct ggttctgggc aataccaaag ataaagtggc ctacctgtgt ctgattaaag
     3721 aagccacctt tgaaaagagt gtggtggccg gtgatcaact ggttattacc agtcgcatta
     3781 cccaaagttt taccccattt tacgtgtttg aagcggttgc caccgtggat agtgaaattg
     3841 ccagtaaagc cgtgattacg ctgagcctga ccattaaagg taccacctaa aggaggtttt
     3901 ctaatgctgg ttacgggtgg tagtaaaggt attggtaaag ccatttgtaa agtgtttatt
     3961 ggtgaaggtg atcatgtgat cgtgaatcat tgtaaaccaa tcgatgatat cgaaaaactg
     4021 cgtgaagaat ttagtagtcc acaaggtagt tttagtattg agaaagccga tgtgaccgat
     4081 tacgcccaaa tcaaacagat ggtggataac attgtgaaaa agcatggtaa aattgatgtg
     4141 ctgattaaca atgcgggcat cattaaggat ggttacatta tgctgatgag tgcgaaagat
     4201 tggaatgatg tgattaatgt gaatctgacc ggtgtgttca attgtaccaa agccgtgagt
     4261 gaacatatga ttggtcaacg tagtggtacg attatcaaca ttgcgagcct gagcggtatc
     4321 acgggtctgc caggtcaaac gaattacagt gccgccaaag gtggtgttat tgcctttacg
     4381 atggccctga gtaaagaact ggccccattt tgtattcgcg ttaatacggt tgccccaggt
     4441 gtgattgaga cggacattat tgatagtctg gataaaaaca aacgtaatag ttacaaagag
     4501 catatcccac tgaaacgctt cggcaaacca gaagaagttg ccagtgtggt tcgttttctg
     4561 gcgagcagtg aagccagcta cattaccggt gaggttattc gtgtgagtgg tggtctgaat
     4621 taatagagat caagccttaa cgaactaaga cccccgcacc gaaaggtccg ggggtttttt
     4681 ttgaccttaa aaacataacc gaggagcaga caggatccta aaagcttcct agg
//
