LOCUS       sequence-160187-         997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an shRNA against NKCC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Young SZ, Taylor MM, Wu S, Ikeda-Matsuo Y, Kubera C, Bordey A
  TITLE     NKCC1 Knockdown Decreases Neuron Production through GABAA-Regulated 
            Neural Progenitor Proliferation and Delays Dendrite Development.
  JOURNAL   J Neurosci. 2012 Sep 26;32(39):13630-8.
  PUBMED    23015452
REFERENCE   2  (bases 1 to 997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2012-09-26"; volume: "32(39)"; pages: "13630-8"
COMMENT     Sequence Label: pSico-shNKCC1
FEATURES             Location/Qualifiers
     source          1..997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     241..261
                     /label=mU6-F
                     /note="Mouse U6 promoter, forward primer"
     protein_bind    complement(279..312)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     enhancer        412..715
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        716..919
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     870..890
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      958..967
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cgctctagag atccgacgcg ccatctctag gcccgcgccg gccccctcgc acagacttgt
       61 gggagaagct cggctactcc cctgccccgg ttaatttgca tataatattt cctagtaact
      121 atagaggctt aatgtgcgat aaaagacaga taatctgttc tttttaatac tagctacatt
      181 ttacatgata ggcttggatt tctataagag atacaaatac taaattatta ttttaaaaaa
      241 cagcacaaaa ggaaactcac cctaactgta aagtaattat aacttcgtat agtataaatt
      301 atacgaagtt ataagccttg gttttttgaa ttccgtatta ccgccatgca ttagttatta
      361 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
      421 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
      481 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
      541 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
      601 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
      661 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
      721 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
      781 tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc
      841 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
      901 ggtctatata agcagagctg gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc
      961 accatggtga gcaagggcga ggagctgttc accgggg
//
