LOCUS       sequence-160189-         859 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses an shRNA against NKCC1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 859)
  AUTHORS   Young SZ, Taylor MM, Wu S, Ikeda-Matsuo Y, Kubera C, Bordey A
  TITLE     NKCC1 Knockdown Decreases Neuron Production through GABAA-Regulated 
            Neural Progenitor Proliferation and Delays Dendrite Development.
  JOURNAL   J Neurosci. 2012 Sep 26;32(39):13630-8.
  PUBMED    23015452
REFERENCE   2  (bases 1 to 859)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J 
            Neurosci."; date: "2012-09-26"; volume: "32(39)"; pages: "13630-8"
COMMENT     Sequence Label: pSico-shNKCC1
FEATURES             Location/Qualifiers
     source          1..859
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(27..60)
                     /label=loxP511
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATAC) (Shaw et al., 2021). loxP511 sites are 
                     compatible with each other, but incompatible with wild-type
                     loxP sites."
     primer_bind     130..146
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     misc_feature    221..809
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(274..294)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(692..703)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(812..828)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ggacgagctg tacaagtagc ggccgcataa cttcgtatag tataaattat acgaagttat
       61 aagccttgtt tgatacactt gtcctgggat tttgatatcc gaatcccagg acaagtgtgt
      121 cttttttctc gaggtcgacg gtatcgataa gctcgcttca cgagatcatg tttaagggtt
      181 ccggttccac taggtacaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa
      241 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
      301 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
      361 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
      421 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
      481 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
      541 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
      601 ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat
      661 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
      721 ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag
      781 tcggatctcc ctttgggccg cctccccgca tcgataccgt cgacctcgat cgagacctag
      841 aaaaacatgg agcaatcac
//
