LOCUS       sequence-160204-         742 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for split sfCherry screening.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Feng S, Sekine S, Pessino V, Li H, Leonetti MD, Huang B
  TITLE     Improved split fluorescent proteins for endogenous protein labeling.
  JOURNAL   Nat Commun. 2017 Aug 29;8(1):370. doi: 10.1038/s41467-017-00494-8.
  PUBMED    28851864
REFERENCE   2  (bases 1 to 742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2017-08-29"; volume: "8(1):370. doi"; pages: " 
            10.1038/s41467-017-00494-8"
COMMENT     Sequence Label: pET_sfCherry(1-10)_32aalinker_sfCherry(11)
FEATURES             Location/Qualifiers
     source          1..742
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(18..35)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     661..679
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ttagcagccg gatctcagtg gtggtggtgg tggtgctcga gttaggtgct gtgtctgccc
       61 tcggctctct cgtactgctc cacgatggtg taagaccccc cgccagcgct ccctccccca
      121 gcagaacctt cccctcccga accagggccc ccgctaccgc ctccttctga tccgccacca
      181 ccaacgtcgt cctcgttgtg gctggtgatg tccagcttga tgtccacgtt gtaggcgccg
      241 ggcagctgca cgggcttctt ggccttgtag gtggtcttca cctcggcgtc gtagtggccg
      301 ccgtccttca gcttcagtct ctggttgatc tcgcccttca gggcgccgtc ctcggggtac
      361 attctctcgg tgctggcctc ccagcccatg gtcttcttct gcatcacggg gccgtcgctg
      421 gggaagttgg tgcccagcag cttcaccttg tagatgaact cgccgtcctg caggctgctg
      481 tcctgggtca cggtcaccac gccgccgtcc tcgaagttca tcactctctc ccaggtgaag
      541 ccctcgggga agctcagctt caggtagtcg gggatgtcgg cggggtgctt cacgtaggcc
      601 ttgctgccgt acatgaactg ggggctcagg atgtcccagg cgaagggcag ggggccgccc
      661 ttggtcacct tcagcttggc ggtctgggtg ccctcgtagg ggtggccctc gccctcgccc
      721 tcgatctcga actcgtggcc gt
//
